Abstract:
In vitro culture of blackgram | Vigna mango (L.) Hepper] cv. LBG was initiated from 7 day
old aseptic seedling shoot tips. Isolated shoot tips were cultured on MS medium containing various
concentrations and combinations of cytokinins and auxins. High frequency of callusing and shoot
differentiation was observed on MS medium supplemented with BAP (3.0 nig 1') and NAA (1.5 mg
1 '1. A single shoot tip produced more than 12 shoots. The regenerated shoots were rooted on MS
medium supplemented with different concentrations of IBA. Seventy five per cent of the shoots
produced r<x>ts, and 90-95% of them survived in the field condition
