Abstract:
Surface sterilisation, stage of explant, media for culture establishment and multiple shoot
induction were standardised for in vitro propagation of rose cv. Folklore. Treatment with mercuric
chloride 0.08% for 12 min was found to be optimum for the surface sterilisation of axillary bud
explants. Axillary buds excised four days after flower opening, having 1.0 cm length, exhibited the
best response in culture establishment. The Murashige and Skoog (MS) basal medium supplemented
with benzyl aminopurine (BAP) 2.5 mg I 1 + 2.4 dichlorophenoxy acetic acid (2,4-D) 0.5 mg 1' was
found to be the suitable medium for culture establishment. The best combination for multiple shoot
induction was MS basal medium supplemented with kinetin 2.0 mg 1' + gibberellic acid (GA,) 1.0
mg 1'.