Abstract:
Plantlets regenerated by in \ilro techniques under high humidity and low temperature in an
artificial medium did not survive by direct planting out in small pots. An improvised structure made of
wooden frame and polyethylene cover helped to maintain high humidity to ex vitro plantlets. Attempts made
to get successful field establishment with different potting media and nutrient solutions did not give any
positive results. Successful hardening and ex vitro establishment of plantlets were achieved by surface
inoculation of different species of vesicular arbuscular mycorrhizae {VAM). Inoculation with Glomus
etunicatum (Becker and Gerd) recorded the highest survival rate of 66.67 per cent, and took minimum days
for flowering.
