In vitro production of toxic metabolite(s) by phytophthora capsic1 and partial purification of the metabolite(s)

Abstract

Phytophthora capsici, the causal organism of Phytophthora foot rot disease in black pepper produces toxic metaholite(s) under in vitro conditions. Maximum accumulation of toxic metabolite(s) was observed in shake cultures of 15 days incubation in Ribeiro's medium. The symptoms induced by toxic metabolite(s) were quite typical to symptoms of natural and artificial infection by the pathogen. The toxic metabolite(s) accumulated in the in vitro culture was found to be heat stable aim non-specific. The toxic metabolite(s) could not be separated using organic solvent fractionation since it is present in the aqueous fraction of the culture filtrate. However, ion exchangers like Dowex 1 and Dowex 50 could be used for separating the metabolite(s) from the aqueous fraction.