Abstract:
A laboratory evaluation and an uptake study using Neubauer seedling technique was carried out for evolving a single extractment suitable for extracting both available P and available K, so as to simplify the process of extracting these available plant nutrients in soil testing works. Eighty seven soil samples from different parts of the State were used to evaluate the performance of the selected number of extractants based on a 3 step evaluation i.e., (i) an initial study consisting one soil, 18 extractants at a single soil solution ratio (1:10) and two equilibration periods (30 and 60 min); (ii) a preliminary screening consisting of 9 soils, 15 extractants at a single soil solution ratio (1:10) and 5 equilibration periods (5, 10, 15, 30 and 60 min). An uptake study was also conducted using Neubauer seedling technique and correlation coefficients were worked out between the amount of P extracted by the various extractants and the P and K uptake by rice; (iii) a final selection of the common extractant consisting of 10 extractants with suitable equilibration periods, at 1:10 soil solution ratio and 87 soils. In combination of NH4 F and DTPA, increasing concentration of NH4 F retarded the extraction of both P and K and they extracted much smaller quantities of P and K as compared to that of Bray No.1 and neutral normal NH4 OAC. Use of ammonium acetate along with Bray No.1 inhibited the release of P drastically. Mathew’s triacid extractant viz., 0.06 N H2 SO4 + 0.06 N HCL + 0.05 N oxalic acid extracted relatively large amounts of P and K. The pattern of P and K release when examined together as a function of period of equilibration found that the equilibration period for different extractants was varying. The suitable equilibration period for 0.1 M NH4F + 0.001 M DTPA, 0.1 M NH4F + 0.003 M DTPA, 0.5 M NH4F + 0.005 M DTPA, 0.05 M NH4F + 0.05 M acetic acid, and Dray No. 1 is 5 min. for 0.3 M NH4F + 0.001 M DTPA combination and Bray No.1 + 0.005 M DTPA an equilibration period of 10 min is found to be the optimum. An equilibration period of 30 min is suitable for Mathew’s triaced extractant, Olsen’s extractant and neutral normal ammonium acetate. Since the initial and preliminary studies established the suitability of Bray No.1 for available P and the neutral normal ammonium acetate for available K and because the coefficient of correlation between the amount of K extracted by the extractants and the K uptake by rice were highly significant at all the equilibration periods the final selection of the common extractant was based on the correlation of the P and K values of the selected extractants with that of Bray No. 1 P and neutral normal ammonium acetate extractable K. The correlation coefficients with Bray No.1 P established by the various extractants have shown the following decreasing order of efficiency. 0.5 M NH4F + 0.005 M DTPA > 0.05 M NH4F + 0.05 M acetic acid > NH4OAC > Olsen > Mathew’s triacid > Bry No.1 + 0.005 M DTPA > 0.3 M NH4F + 0.001 M DTPA > 0.1 M NH4F + 0.001 M DTPA > 0.1 M nH4F + 0.003 M DTPA. All the extractants were not significantly and positively correlated with NH4 OAC (std) K. The three extractants viz., Mathew’s triacid, 0.1 M NH4F + 0.001 M DTPA and Olsen’s extractant have given significant positive relationship with NH4 OAC (std) K. Highest correlation was obtained with Mathew’s triacid followed by 0.1 M NH4F + 0.001 M DTPA and Olsen’s extractant. Thus it is confirmed that Mathew’s triacid extractant is the best common extractant for available P and available K. No analytical difficulty in the determination both P and K was observed while using this extractant. Inter-correlations worked out between the various soil properties have cleared the following facts. Mathew’s triacid P and Bray No.1 P were significantly and positively correlated with total P and negatively correlated with P fixing capacity. NH4 OAC (std) K and Mathew’s triacid K gave significant positive correlation with total K as well as CEC. Mathew’s triacid extractant viz., 0.06 N H2SO4 + 0.06 N HCL + 0.05 N oxalic acid with a soil solution ratio of 1:10 and an equilibration period of 30 min is recommended as a commom extractant for available P and available K by the present study, since it saves considerable time and materials in soil testing.