Crops loss caused by root-knot nematode infesting coleus parviflours and its control

Abstract

Crop loss caused by the root-knot nematode infesting Coleus parviflorus and its control was studied in a pot culture experiment and a field experiment in the Department of Entomology, College of Agriculture, Vellayani, Trivandrum, during August to December, 1986. The number of leaves, shoot length, fresh shoot weight and weight of tubers obtained were less in inoculated plants than in control. The yield and growth parameters showed an negative correlation with the population of nematodes. A high positive correlation was noticed between the tuber weight and the number of leaves and shoot weight of coleus. The shoot length did not influence the tuber yield significantly. Roots showed profuse galling by the second month. Rotting of roots was observed by the third month, and at harvest, no healthy root was present in infected plants. Tubers when stored developed dark patches on the suface of galls and these spread inwards covering the whole of the internal tissues within nine days, and by the twelfth day the internal contents had turned into a dark brown watery liquid with a bad odour. The peel of the tubers did not show rotting. The nature of rot indicated the involvement of secondary organisms like bacterial and fungi. Histopathological studies showed that the nematodes were lodged in the cortex with head in the stellar region of the root. One month after inoculation, quadrangular giant cells (four in number) were observed in roots. There was cell proliferation in the stellar region of the infected roots. Alternate arrangement of the xylem and phloem was disturbed, though the central cylinder and cortex could be distinguished. Two months after inoculation the giant cells were seen larger and fewer in number. The cytoplasm of giant cells became granular and aggregated towards the head region of the nematode. Stele could not be easily distinguished from the cortex. The cortex was greatly enlarged due to hypertrophy and hyperplasia of cells. The development of medullary rays and secondary xylem noticed in the normal root could not be distinguished in infected roots. Infected roots showed numerous cavities formed by the disintegration of giant cells, egg masses and adult females by end of the fourth month. Necrosis of tissue was also observed. The xylem and phloem elements were not distinguishable. At five months after inoculation the tissues of the roots were completely rotten. In the field experiment application of nematicides one month after planting preceded by the burning of plant material in the field prior to planting was found better than the other treatments. Carbofuran @ 1 kg a.i./ha was more effective than phorate for the control of the nematode. No residue of carbofuran or phorate could be detected in tubers at harvest. The rotting of tubers obtained from different plots when kept in store indicated the inefficacy of the treatments for giving protection from the nematode and the need for a second application of the nematicide at tuber setting. Hot water treatments of the tubers prior to storage did not reduce the damage.