Abstract:
An investigation was undertaken to study the uterine pathology in repeat breeding cows employing uterine biopsy as a diagnostic technique. The studies were carried out in 26 repeat breeding cross bred cows from the herd of University Livestock Farm, Mannuthy using a biopsy instrument which was a modification of one designed by Minocha et al. (1964). Four cows which settled at first insemination served as control animals for comparison. From all the animals biopsy was taken during the early part of heat and was inseminated 8 to 12 hours after biopsy followed by intrauterine antibiotic therapy after 12 to 24 hours. Seven out of 17 animals which failed to conceive even after biopsy and treatment were slaughtered and their genitalia were subjected to detailed studies.
Nine out of 26 repeat breeders and all the four control animals conceived at inseminations in the same heat of biopsy explaining the fact that biopsy operation did not affect the functional status of uterus. Pathological changes in the endometrium were observed in 14 out of 26 (53.85%) repeat breeding cows. Changes in the uterus observed in the biopsy were significant and consisted of infiltration with various types of inflammatory cells, periglandular fibrosis, cystic dilatation of glands, glandular hypertrophy, stromal hyalinization and sclerosis. It was seen that endometritis constituted the most important lesion.
The experimental animals were classified into three groups based on histological findings; group I showing normal or nearly normal endometrium, group II with more extensive inflammatory changes and group III with severe inflammatory and fibrotic changes. Group I and II had a conception rate of 58.33 and 25 per cent respectively. In group III, none conceived. Results of insemination revealed that uterine lesions characterized by severe cellular infiltration and glandular changes affected the changes of conception adversely. The lesions in the slaughtered animals correlated with biopsy findings in general.
