Evaluation of seed source variation and clonal propagation techniqes in Jatropha curcas linn.

Abstract

A study was conducted in College of Forestry, Vellanikkara, Trichur, during the period 2005-2007 to evaluate different seed sources Jatropha curcas, a potential source of producing biodiesel for their genetic variation and to standardize efficient clonal propagation techniques. The study involved the evaluation of seed sources for their seed and seedling parameters as well as field performance of the plants. Attempts were made to standardize of macro and micro propagation techniques for the multiplication of elite genotypes.

The material used for the evaluation consisted of different seed sources from various parts of Kerala and Karnataka. Although the variation among the seed sources for most of the seedling characters in the nursery was found non significant, a considerable variation was observed among them in their field performance. Considerable variation was also noticed for the seed parameters such as length, width, 100 seed weight, germination percentage, kernel: seed weight ratio and seed oil content. None of the seed sources excelled for all the characters studied. Among the different seed sources three seed sources viz., Kasargod, KAU and Palakkad seed sources were found to be superior in terms of most of the characters studied.

Standardization of rooting of cuttings was attempted with 10 and 20 cm cuttings taken from semi-hardwood and softwood parts of the stem. Three levels each of IAA, IBA and NAA were used for evaluating their efficiency for rooting as well as shoot formation in the stem cuttings. Effect of these hormones on the shoot parameter was found less significant. However, highest sprouting was recorded in IAA 100 ppm where as IAA 250 ppm recorded highest number of shoots. Almost all the root characters were found to be greatly influenced by growth hormones. The semi-hardwood cuttings were found superior to the soft wood cuttings while, the 20 cm cuttings were found better than 10 cm cuttings with respect to most of the characters studied.

Clonal propagation of Jatropha curcas was attempted by micro propagation through tissue culture using nodal segments as explants. Among the three basal media tried viz., MS, WPM and B5 medium, MS was found to be better in terms of bud, leaf and shoot initiation. The culture establishment was greatly influenced by the season of culturing. All explants cultured during the rainy season were got contaminated. A fungicidal dip in 0.2 per cent Bavistin (Carbendazim) and Indofil M- 45 (Mancozeb) for 1 hour followed by 15 minute dip in 0.1 per cent HgCl2 was the most effective surface sterilization procedure. Murashige and Skoog medium supplemented with 1.0 mg l-1 kin was found to be the best medium for shoot production. Highest average number of leaves (3.47), maximum number of leaves (8.67) and maximum shoot length was observed in this medium. The synergistic effect of BA and kin in MS medium was found to be better than supplementing them individually especially for the enhanced release of axillary buds. The treatment MS+0.5 mg l-1BA+1.0 mg l-1 kin was found to be the best treatment combination to get highest shoot initiation.