Browsing by Author "George, M C"
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Item Ensiling cuttlefish wastes for use as fish feed ingredient(Department of Processing Technology, College of Fisheries, Panangad, 1999) Santhosh Kumar, C; George, M CThe disposal of cuttle fish processing waste is becoming a major problem for the seafood processing industries in India. Formic acid fish silages and fermented fish silages were prepared using the waste. The silage preparations were stored for 90 days. Samples were drawn on 0,2,3,5,7,14,30,60 and 90th days of storage. The pH and various biochemical parameters like, total nitrogen (TN), non protein nitrogen (NPN), total volatile base nitrogen (TVBN), tri methyl amine (TMA) and peroxide value (PV) were carried out. Microbial analyses, viz., total plate count (TPC) and most probable number (M.P.N) of coliforms were carried out for formic acid fish silage preparations, whereas for fermented fish silage preparations, acid producing bacterial count (APBC) and M.P.N of coliforms were determined. All the combinations of fermented fish silage preparations studied, preserved well. A pH of not more than 3.8 was necessary for the formic acid fish silage to be preserved for 90 days. All the silages, which preserved well, were used for making fish feed. The pelletised feeds were fed to common carp tCyprinus carpio) fingerlings. The feeds were consumed by the fishes within 15 minutes of feeding.Item Immunology survey on the incidence of infectious bronchitis(IB) and infectious laryngotracheitis (ILT) in poultry in and around Trichur(Department of Microbiology, College of Veterinary and Animal Sciences, Mannuthy, 1979) George, M C; Punnoose, K TInfectious bronchitis and infectious laryngotrancheitis are the two viral diseases of poultry responsible for economic loss to the poultry industry by way of decreased egg production, poor quality of eggs, decreased feed efficiency and loss of weight gain. These disease have been reported from the neighbouring states of Kerala. In the present study a serological survey was carried out to understand the prevalence of these two disease in the poultry population in and around Trichur. A total of 2,110 serum samples have been collected from the field, comprising of white leghorn, Rhode Island Red and Desi birds belonging to different age groups. Serum samples were collected from organized farms, from birds kept by farmers and from the birds slaughtered in different hotels at Trichur. These serum samples were tested against the infectious bronchitis and infectious laryngotracheitis by employing agar gel precipitation test. The chorioallantoic membrane and allantoic fluid of infected embryos were used for the preparation of antigens for agar gel precipitation test. The potency of antigens was tested by conducting the agar gel precipitation test with corresponding hyper immuns sera prepared in white leghon male chicks of six to eight weeks of age. A line of precipitation was obtained in both cases which was close and curved towards the antigen well, because of the high concentration of antibody in the sera and due to the high molecular weight of the antigen. In the case of infectious bronchitis the line of precipitation was distinct where as in case of infectious laryngotrachetis it was diffused. The antigen, whose efficiency was tested using hyper immune sera, was used to test samples of sera collected from the field. The samples were pooled to 211 groups and tested for the presence of infectious bronchitis and infectious larygotracheitis precipitating antibodies separately by agar gel precipitation test. None of the samples gave precipitin line either to infectious bronchitis or to infectious larygotracheitis. So it was assumed that both of these viral disease are not prevalent in Trichur and its suburbs.