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Sensor based nutrient management in Tomato (Solanum lycopersicum)
(Department of Soil Science and Agricultural Chemistry, College of Agriculture, Vellayani, 2026-02-09) Mayoora Shaji; Rekh, V R Nair
The study entitled ‘Sensor based nutrient management in tomato (Solanum
lycopersicum)’ has been carried out at the Department of Soil Science and Agricultural
Chemistry, College of Agriculture, Vellayani, during 2023-2025. The objective of the
study was to compare soil test values obtained through different sensors and laboratory
analysis; Yield assessment to analyse the efficiency of the sensor using tomato as the
test crop.
The study was carried out in two parts. The first part focused on comparing
nutrient analysis across different cropping systems using sensor-based measurements
and conventional laboratory analysis. Surface soil samples (0-15 cm) were collected
from four land-use systems, , vegetable-based cropping system, coconut-based mixed
farming, homestead farming, and fallow land within AEU 8. Five samples were
collected from each system using the standard soil sampling procedure, with one
composite sample taken for every 20 cents of area. Two portable sensors were deployed
to measure soil nutrients at each location. Sensor 1 (Modbus) provides primary nutrient
estimates (N, P, K) using an electrical-conductivity-based sensing principle. Sensor 2
(NutRE) measures primary (N, P, K), secondary nutrients (Ca, Mg, S), and selected
micronutrients (Fe, Zn, Cu) through an ion-sensing mechanism, with further technical
details withheld due to a pending patent.
For field measurements, six random sampling points were selected within each
20-cent area, matching the locations used for conventional soil sampling. Modbus
readings were taken at two points and averaged, and this procedure was repeated across
the remaining 3-6 randomly selected spots within each location. The same sampling
approach was applied for the NutRE sensor. The mean nutrient values obtained from
both sensors were then compared with corresponding laboratory measurements. The
Modbus sensor (Sensor 1) showed no significant difference (p > 0.05) for P under all
land-use systems and across all computed mean levels (M2—M6), indicating close
agreement with laboratory values for these parameters. However, N and K showed
significant deviation (p = 0.00), suggesting the need for calibration for these nutrients.
In contrast, the NutRE sensor demonstrated non-significant differences (p > 0.05) for
all measured nutrients (N, P, K, Ca, Mg, S, Fe, Zn, Cu) across all land-use systems and
mean levels, reflecting strong alignment with laboratory analysis. Variation in the
number of readings used for mean calculation (M2—M6) did not significantly influence
sensor accuracy for either sensor. Therefore, adopting the minimum mean level (M2)
was adequate for accurate nutrient estimation across all systems, improving operational
efficiency by reducing sampling time and field effort.
The second part involved a field experiment that assessed sensor efficiency
through tomato (cv.Anagha) yield evaluation, where the performance of both sensors
was compared against soil test-based nutrient management. The experiment consisted
of five treatments: T: (STB) — Split application of N P K as per soil test data (laboratory
analysis). Tz (MDS-Nb) — Split application of N P K as per sensor | reading at 15 days
interval up to beginning of harvest (Modbus sensor without basal). Ts (NutRE-Nb) —
Split application of N P K as per sensor 2 reading at 15 days interval up to beginning of
harvest (NutRE sensor without basal). Ts (MDS-B) — NPK application as basal dose
based on Ist sensor data and further split up at 15 days interval (Modbus sensor with
basal). Ts (NutRE-B) — NPK application as basal dose based on 2nd sensor data and
further split up at 15 days interval (NutRE sensor with basal).
Biometric observations revealed, at harvest, T: recorded the highest plant height
(61.17 cm), while Ts produced a significantly greater number of branches (4.81). Leaf
Area Index was highest under T; (2.59). With respect to yield attributes, T: produced a
significantly greater number of fruits per plant (34.81), and the maximum fruit yield per
plant (1.10 kg) while Ts (35.65g) produced highest fruit weight. Post-harvest soil
analysis revealed significantly higher available N (210.12 kg ha‘) and K (207.20 kg
ha"') under Ti, whereas Ts recorded the highest available P (85.20 kg ha‘). Plant
nutrient analysis conducted at 15 and 30 DAT showed that Ts consistently recorded
maximum NPK content during early crop growth, while at harvest, N and K contents
were highest in T, and P content was highest in Ts. A similar trend was observed in
nutrient uptake. Economic analysis showed that Ti yielded the highest gross income
(Rs. 720,254.80) and B:C ratio (1.81), closely followed by Ts.
T-test and Absolute Error Percentage (AEP) results showed that NutRE sensor
accurately estimated all nutrients, with non-significant differences from laboratory
values. Modbus sensor was reliable for phosphorus, while nitrogen and potassium
required calibration to match laboratory standards. Field experiments revealed that soil
test-based nutrient management T: (STB) achieved the highest yield, which was
statistically comparable to the calibrated NutRE-based treatment Ts (NutRE-B). These
results confirm that sensor-based nutrient management becomes effective only when
supported by proper calibration and the inclusion of basal nutrient doses.
Influence of fundamental motives on retention of certified organic farmers in Kerala agricultural development society, Thodupuzha
(College of Co-operation, Banking and Management, Vellanikkara, 2026-09-12) Devika, C G; Biju Thomas Muttath
Awareness and adoption of farm mechanisation by rice farmers in Palakkad district
(College of Co-operation,Banking & Management, Vellanikkara, 2023-09-13) Thomson James; Divya Vijayan
Etiology and management of leaf spot disease of arecanut in northern Kerala By: Bhavana, A S
(Department of Plant Pathology, College of Agriculture, Padannakkad, 2026-02-19) Bhavana, A S; Sajeesh, P K
The study entitled “Etiology and management of leaf spot disease of
arecanut in Northern Kerala” was carried out in the Department of Plant
Pathology, College of Agriculture, Padannakkad during 2023-2025 with the
objective of etiology of the fungal pathogen/s associated with the leafspot disease
of arecanut and to formulate management strategies against the disease.
A purposive sampling survey was conducted in four AEUs of Kasaragod
and Kannur districts to collect infected and healthy leaf of arecanut leaf spot
disease affected palms samples. The fungi associated with the leaf spot
symptoms were isolated and characterised. Pathogenicity tests revealed that
twelve isolates were pathogenic, producing symptoms within 12–15 days after
inoculation. These twelve pathogenic isolates were subsequently sent to the Rajiv
Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram, for molecular
characterization. Among the six isolates three isolates showed high similarity
with Colletotrichum arecicola which is reported for the first time on arecanut in
India.
The contents of nitrogen (N), potassium (K), calcium (Ca), magnesium
(Mg), and boron (B) were analysed in both healthy and diseased leaf samples
collected from various locations and AEUs. A total set of healthy and diseased
leaf samples were analysed using standard protocols. It was found that K level
was consistently higher in healthy samples compared to diseased ones, indicating
the role of K in enhancing disease resistance. Nitrogen and magnesium showed
variable patterns depending on the AEU and did not follow a uniform trend.
Calcium content was generally higher in healthy leaves, except in the leaves
collected from AEU 13. Boron levels tended to be higher in diseased leaves
suggesting a possible stress induced accumulation.
Phylloplane microorganisms were isolated from the healthy arecanut
leaves. These were evaluated for antagonism towards Colletotrichum arecicola
using dual culture technique. Among them, two Trichoderma- like fungal
isolates and one-gram negative bacterial isolate exhibited strong inhibition of
the predominant pathogen C. arecicola. Under in vitro evaluation of fungicides
against C. arecicola by poison food technique, mancozeb (75WP) at 0.2%,
propiconazole (25EC) at 0.2% and carbendazim 12% + mancozeb 63% (75
WP) at 0.2% exhibited high inhibition (100%). These three fungicides, along
with the antagonists selected from phylloplane used for in vivo evaluation
against the disease. Fungicides and antagonists were applied one week after
pathogen inoculation on leaves. After 15 days of fungicide application,
propiconazole (25 EC) at 0.2% and carbendazim 12% + mancozeb 63% (75
WP) at 0.2% were found effective against arecanut leaf spot.
The study identified as C. arecicola is the major cause of arecanut leaf
spot in Northern Kerala, and it reported for the first time on arecanut in India.
Nutrient analysis showed that higher potassium levels were associated with
healthier leaves. Among the management options tested, propiconazole (25 EC)
at 0.2% and carbendazim 12% + mancozeb 63% (75 WP) at 0.2% were the most
effective in reducing disease severity. The findings are helped to learn more
about the etiology of the leaf spot disease and in developing improved fungicide
and fertilizer schedules for managing arecanut leaf spot disease.
Characterization of pathogens causing canker and dieback diseases in moringa (Moringa oleifera Lam.)
(Department of Plant Pathology, College of Agriculture, Vellanikkara, 2026-02-06) Adithya Sreekumar; Anju, C
Canker and dieback are increasingly recognized as major constraints in moringa
cultivation, despite the crop’s well-known resilience and rich phytochemical defence.
Field observations across Kerala have indicated a rising incidence of these diseases
affecting young and mature plants alike, yet systematic documentation of the causal
pathogens and their interaction with the host remains limited. The present investigation
was undertaken to identify, characterize and evaluate the pathogenic potential of fungi
associated with canker and dieback diseases of moringa, and further to assess host
biochemical responses at critical developmental stages. The study was carried out in the
Department of Plant Pathology, College of Agriculture, Vellanikkara, from 2023 to
2025.
A purposive survey of moringa-growing homesteads and nurseries in Thrissur
(AEU 10) and Ernakulam (AEU 12) districts yielded a total of 65 diseased samples
from which 77 fungal isolates were obtained, comprising 50 from canker-affected
tissues and 27 from dieback samples. Disease intensity varied among locations, with
the highest PD1 for canker recorded at Kolazhy (AEU 10) (89.28 %), while the
maximum PD1 for dieback was recorded at Kothamangalam (AEU 12) (76 %). Several
samples yielded multiple isolates, indicating the coexistence of distinct fungal species
within the same lesion. Isolates were purified using hyphal-tip culture and maintained
on PDA for further studies. Canker symptoms under natural conditions included
superficial bark cracking, pale-yellow to light-brown sunken or flat lesions and
localized necrosis. Dieback symptoms, on the other hand, were characterized by
progressive twig/branch drying, extensive cortical discoloration and the appearance of
black, pinhead-like pycnidial structures on severely affected tissues. Pathogenicity
testing conducted under rain-shelter conditions confirmed the ability of all tested
isolates to reproduce disease symptoms on moringa, fulfilling Koch’s postulates.
Symptom development varied markedly among isolates, indicating a wide spectrum of
virulence.
Cultural and morphological characterization of the isolates revealed
considerable diversity in colony growth, pigmentation, sporulation and microscopic
traits. Isolates associated with canker generally produced fast-spreading, cottony to
fluffy mycelium with pink, white, or grey colouration, while dieback-associated isolates
commonly showed dark, melanised mycelium with abundant pycnidial formation.
Molecular characterization using ITS, TEF-1α and RPB2 gene regions confirmed the
presence of multiple pathogenic species on moringa. Canker symptoms were associated
with Fusarium irregulare, F. citri, Nectria sp. and Lasiodiplodia theobromae, whereas
dieback was predominantly caused by L. theobromae, Curvularia sp. and Diaporthe
batatas. This represents the first comprehensive documentation of the canker-dieback
pathogen complex occurring on moringa in Kerala.
Extracellular cellulases and pectinases production was evaluated using
substrate-amended media. All isolates exhibited measurable cellulolytic and
pectinolytic activity. Among the 77 tested isolates, several, including Fusarium sp.
(AIS5), Fusarium sp. (VLD2), Lasiodiplodia sp. (VLD1), Fusarium sp. (MAD),
Curvularia sp. (MLD2) and Curvularia sp. (AVD3), produced large hydrolytic zones
(mm) within just 2-3 days, reflecting a strong enzymatic activity. Statistical analysis
2
revealed a highly significant positive correlation between lesion size and enzyme
activity, indicating that both cellulase and pectinase secretion are strongly linked to
virulence expression.
Two highly virulent isolates, L. theobromae, CHS2 (canker) and L. theobromae,
TRD1 (dieback), were selected for biochemical assays. Host response was examined at
five principal developmental stages: leaf, shoot, flowering, fruiting and senescence.
CHS2 produced small, slowly enlarging lesions across all developmental stages,
beginning as light-brown or pale-yellow spots (0.1-0.2 cm) that expanded slowly and
developed into ovoid to spindle-shaped forms with mild cracking by day five. In
contrast, TRD1 generated faster-expanding lesions even in early stages, initial pale
brown patches (0.1-0.2 cm) rapidly enlarged (up to 2.5 cm by day 5) and frequently
coalesced, progressing from pale yellow or water-soaked patches to darker brown or
black lesions. Overall, CHS2 showed slower, stage-dependent lesion expansion,
whereas TRD1 exhibited rapid, aggressive lesion development across all growth stages.
Total phenolic content varied widely among growth stages and according to
pathogens. In the flower development stage, inoculated plants accumulated
substantially higher phenols than controls from one day after pathogen inoculation,
indicating a pronounced early defence response. Conversely, in leaf and fruit
development stages, phenol levels in inoculated plants dropped significantly below
those of the corresponding controls, suggesting growth stage-specific phenol
biosynthesis in response to pathogen infection. During senescence, phenol levels
remained low across TRD1 inoculated treatment, reflecting a natural decline in defence
capacity. Flavonoid content showed a transient decline at one to two days post
inoculation across most developmental stages. This was followed by a clear rebound on
days three and five, especially in the leaf and shoot development stages, reflecting
induced defence activation. In the leaf development stage inoculated with CHS2,
flavonoids increased markedly by day five (22.85 mg QE g⁻¹; ~26% above control).
TRD1-inoculated tissues displayed a similar pattern of early reduction followed by
strong accumulation of flavonoids from day three.
Overall, the study establishes that canker and dieback in moringa are caused by
a diverse assemblage of fungal pathogens exhibiting wide variability in virulence and
extracellular enzyme activity. The findings highlight the central role of cell-wall
degrading enzymes in facilitating tissue colonization and disease progression.
Moreover, phenolic and flavonoid profiling across growth stages reveals that moringa
exhibits a highly stage-dependent defence strategy, with stronger biochemical activation
during early vegetative growth and flowering stages and weaker responses during
fruiting and senescence. These insights form an essential foundation for developing
stage-specific disease management strategies and for future exploration of host
resistance mechanisms in moringa.