Browsing by Author "Reshmika, P K"

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    Development of inbreds in bitter gourd (momordica charantia L.) through conventional and biotechnological approaches
    (Department of Vegetable Science, College of Horticulture, Vellanikkara, 2020) Reshmika, P K; Pradeepkumar, T
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    Varietal responses of rootstock to epicotyl and softwood grafting in Kuttiattoor mango
    (Department of Fruit Science, College of Agriculture,Padannakkad, 2025) Suchitra, B.; Reshmika, P K
    An investigation was undertaken to validate the influence of rootstock and its age on epicotyl and softwood grafting in Kuttiattoor mango at the Department of Fruit Science, College of Agriculture, Padannakkad during the year 2023-24. The experiments were laid out in factorial arrangement using CRD with two replications. The first experiment aimed to validate the influence of rootstock and its age on epicotyl grafting in Kuttiattoor mango which include rootstocks of Vellaikolumban (Vel), Kurukkan (Kur), Kalapady (Kal) and Kuttiattoor (Kut) with each rootstock cultivar used at three different ages: seven days old (7D), 10 days old (10D) and 13 days old (13D). The main effect of rootstock cultivars on GSPs indicated that Kuttiattoor grafts with Kal rootstocks performed the best in terms of percentage of sprouting (82.33%), percentage of survival (52.95%) and days required to graft sprouting (12.35), followed by the grafts with Kut, Kur and Vel rootstocks. The main effect of age of rootstock cultivars indicated that grafting performed on 7D rootstock yielded good results for all GSPs. As per the interaction effects, Kuttiattoor grafts with Kal 7D rootstocks recorded the highest percentage of sprouting (91%), percentage of survival (61.37%) and the minimum days required to graft sprouting (11.3) which was statistically on par with the performance of grafts with Kut 7D rootstocks. Observations on GGPs were recorded at fortnightly intervals up to 98 days after grafting (DAG). The main effect of rootstock cultivars revealed that Kuttiattoor grafts with Kal rootstocks showed the highest for all GGPs at all intervals which was statistically similar to the performance of grafts with Kut at certain intervals, followed by those with Kur and Vel rootstocks. The main effect of rootstock age indicated that grafts with 7D rootstocks achieved the highest for all the GGPs throughout the experiment, followed by 10 D and 13 D rootstocks. Interaction effects revealed that grafts with Kut 7D and Kal 7D rootstocks were comparable for most of the GGPs at various intervals. At 98 DAG, the Kutiattoor grafts with Kal 7D rootstocks showed maximum value for GGPs including elongation of scion (13.85cm), number of leaves (18.8), girth of new growth (2.16cm), girth of scion (3.34cm), girth of stock (3.44cm) and number of primary branches (2.55). This performance was statistically on par with that of Kut 7D for all GGPs except for girth of new growth. Grafts with Vel 13D rootstocks showed the minimum value for all GGPs throughout the experiment, which was on par with Kur 13 D rootstocks for most of the intervals. The higher activity of fascicular and interfascicular cambial cells resulted in enhanced callus formation and quicker healing of graft union, which likely resulted in a higher success rate for epicotyl grafting. The Kuttiattoor grafts with Kal 7D rootstocks manifested relatively efficient callus growth and vascular differentiation, followed by those with Kut 7D rootstocks. The graft union, callus formation and vascular differentiation in grafts with Vel 13D rootstocks followed by Kur 13D rootstocks progressed at a slower pace. The second experiment aimed to validate the influence of rootstock and its age on softwood grafting in Kuttiattoor mango which include rootstocks of Vel, Kur, Kal and Kut with each rootstock cultivar used at three different ages: one month old (1M), two months old (2M) and three months old (3M). The main effect of rootstock cultivars on GSPs indicated that Kuttiattoor grafts with Kut rootstocks performed the best in terms of percentage of sprouting (73%), percentage of survival (41.92%) and days required to graft sprouting (12.50), followed by those with Kal, Kur and Vel rootstocks Grafting performed on rootstock at three months of age gave good results for all GSPs. As per the interaction effects, Kuttiattoor grafts with Kut 3M performed well in terms of days to graft sprouting (11.5), percentage of sprouting (85 %) and survival (47.11%) followed by grafts with Kal 3M. Observation on GGPs were recorded at fortnightly intervals up to 140 DAG. The main effect of rootstock cultivars revealed that Kuttiattoor grafts with Kut rootstocks observed the highest for all GGPs at all intervals. This was statistically similar to the performance of grafts with Kal at certain intervals, followed by those with Kur and Vel rootstocks. The main effect of rootstock age indicated that grafts with 3M rootstocks achieved the highest for all GGPs throughout the period of observation, followed by those with 2M and 1M old rootstocks. Interaction effects indicated that grafts with Kut 3M and Kal 3M rootstocks were comparable for most of the GGPs at various intervals. They performed statistically similar for girth of new growth across all intervals. At 140 DAG, grafts with Kut 3M showed the highest value for GGPs including scion elongation (18.99cm), number of leaves (24.9), girth of new growth (2.51cm), girth of scion (3.61cm), girth of stock (3.69cm) and number of primary branches (2.9). This performance was on par with that of Kal 3M. Grafts with Vel 1M rootstocks recorded the minimum value for all GGPs throughout the period of experiment, which was on par with Kur 1M rootstocks for most of the intervals. The histological studies revealed that the Kuttiattoor grafts with Kut 3M rootstocks showed the most consistent vascular connections and advanced callus formation followed by those with Kal 3 M rootstocks. The grafts of Kuttiattoor on Vel 1M rootstocks followed by Kur 1M rootstocks showed a slower rate of graft union development, callus formation and vascular connectivity.