Genetic diversity and domestication of Pyrenacantha volubilis Wight, an anti cancer drug yielding plant
By: Arjun Ramachandran.
Contributor(s): Santhoshkumar, A V (Guide).
Material type:
BookPublisher: Vellanikkara Department of Forest Biology and Tree Improvement, College of Forestry 2020Description: 106p.Subject(s): Forest biology and tree improvementDDC classification: 634.9 Online resources: Click here to access online Dissertation note: PhD Abstract: The present study titled “Genetic diversity and domestication of Pyrenacantha
volubilis Wight: an anti-cancer drug yielding plant” was carried out from 2017 to
2020 with the objectives of exploring the genetic diversity in fragmented natural
populations of P. volubilis, analyzing camptothecin and its major derivatives
isolated from P. volubilis for druggability against major oncoproteins, identifying
elite lianas through a progeny trial and propagating the plant vegetatively. UPGMA
classified the 12 natural populations of P. volubilis into seven distinct classes based
on the amplicon profiles of the 12 ISSR primers considered for the study. There is
high genetic diversity among populations of P. volubilis, the full potential of which
must be tapped for domestication. The binding energy computed for interactions of
camptothecinoids with oncoproteins and Topo I were unstable because
camptothecinoids act on the ‘cleavable complex’. 20 three-year-old lianas (PV01
to PV20) superior for vegetative and reproductive growth traits were screened by
progeny evaluation. CPT accumulation in vegetative parts was in trace amounts for
all the nine progenies tested in this experiment. The maximum harvest weight of
seeds per plant was for progeny PV10-01 (4.57g). The concentration of
camptothecin (2.12%) coupled with prolific seed bearing (9 seeds per plant) after
the first year itself makes PV10-01 superior to all other progenies for overall
camptothecin yield. Results of the progeny trial indicate that mother plants PV01,
PV02, PV06, PV08 and PV10 may be considered for higher economic gains. Four
to eight noded stem cuttings (softwood, semi-hardwood, hardwood) were treated
with IBA (0, 2000, 4000, 6000, 8000 and 10000mgL-1) and laid out in a 3×6
Factorial CRD in a semi-automatic mist chamber to standardize vegetative
propagation. Hardwood cuttings treated with 2000mgL-1 (83.33%) and 4000mgL-1
(75.56%) IBA and softwood cuttings treated with 4000mgL-1 (60.12%) IBA were
superior for rooting percentage.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | Reference Book | 634.9 ARJ/GE PhD (Browse shelf) | Available | 175036 |
PhD
The present study titled “Genetic diversity and domestication of Pyrenacantha
volubilis Wight: an anti-cancer drug yielding plant” was carried out from 2017 to
2020 with the objectives of exploring the genetic diversity in fragmented natural
populations of P. volubilis, analyzing camptothecin and its major derivatives
isolated from P. volubilis for druggability against major oncoproteins, identifying
elite lianas through a progeny trial and propagating the plant vegetatively. UPGMA
classified the 12 natural populations of P. volubilis into seven distinct classes based
on the amplicon profiles of the 12 ISSR primers considered for the study. There is
high genetic diversity among populations of P. volubilis, the full potential of which
must be tapped for domestication. The binding energy computed for interactions of
camptothecinoids with oncoproteins and Topo I were unstable because
camptothecinoids act on the ‘cleavable complex’. 20 three-year-old lianas (PV01
to PV20) superior for vegetative and reproductive growth traits were screened by
progeny evaluation. CPT accumulation in vegetative parts was in trace amounts for
all the nine progenies tested in this experiment. The maximum harvest weight of
seeds per plant was for progeny PV10-01 (4.57g). The concentration of
camptothecin (2.12%) coupled with prolific seed bearing (9 seeds per plant) after
the first year itself makes PV10-01 superior to all other progenies for overall
camptothecin yield. Results of the progeny trial indicate that mother plants PV01,
PV02, PV06, PV08 and PV10 may be considered for higher economic gains. Four
to eight noded stem cuttings (softwood, semi-hardwood, hardwood) were treated
with IBA (0, 2000, 4000, 6000, 8000 and 10000mgL-1) and laid out in a 3×6
Factorial CRD in a semi-automatic mist chamber to standardize vegetative
propagation. Hardwood cuttings treated with 2000mgL-1 (83.33%) and 4000mgL-1
(75.56%) IBA and softwood cuttings treated with 4000mgL-1 (60.12%) IBA were
superior for rooting percentage.
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