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Anaesthetization of Liza Parsia (Hamilton) Fry for Oxygen - Packed Transportation

By: Vimala Rose Mathew.
Contributor(s): Jayasree Vadhyar K(Guide).
Material type: materialTypeLabelBookPublisher: Panangad Department of Aquaculture, College of Fisheries 1998DDC classification: 639.2 Online resources: Click here to access online | Click here to access online Dissertation note: MFSc Abstract: Three fish anaesthetics viz, chloral hydrate at 0.20, 0.25 and 0.30 g/l, tertiary butyl alcohol at 2.5, 3.0 and 3.5 m1!1 and clove oil at 8.5, 9.0 and 9.5 mg/l were tried on L parsia fry of average weight 0.22 g, at 7 ppt salinity and at ambient temperature 28 ± 2°C to determine the optimum dose of each. The optimum dose was selected on the basis of behavioural pattern, induction and recovery times, ra.e of oxygen consumption and post-treatment survival. The optimum doses obtained for chloral hydrate, tertiary butyl alcohol and clove oil were 0 J 0 g/l, 2.5 mlll and 8.5 mg/l respectively. The effect of each anaesthetic at the selected dose on the metabolic rate of the fry was compared, and significant difference was observed amongst the three anaesthetics and control at these doses. Chloral hydrate and clove oil treatment resulted in lower oxygen consumption rate than that of tertiary butyl , '..11:' _ O;-=-, : ••• - '.' .- • ..c:' alcohol treatment No significant effect was observed on the percentage survival and duration of the fry treated with the three anaesthetics at the selected doses in the oxygen- packed jars. Packing density had significant effect on the sur vival and duration of oxygen-packed fry. The safe duration with 100% survival was 15. ,6 h at 100 fry/I and 3 to 7 hat 200, 300, 400 and 500 fry/I. Linear relationships could be established in the case of (1) chloral hydrate treatment, in the oxygen-packed jars at 70% survival between (a) packing density and dissolved oxygen levels, (b) packing density and carbon dioxide levels and (c) packing density and ammonia -N levels and (2) for tertiary butyl alcohol treatment 91 between (a) packiruz density and dissolved oxygen levels and (b) packing density and carbon dioxide levels Significant difference was observed in the carbon dioxide levels between treated and control jars, while no significant difference was noted in the dissolved oxygen and ammonia -N levels in the treated and control jars
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Theses
639.2 VIM/AN (Browse shelf) Available 171439

MFSc

Three fish anaesthetics viz, chloral hydrate at 0.20, 0.25 and 0.30 g/l, tertiary
butyl alcohol at 2.5, 3.0 and 3.5 m1!1 and clove oil at 8.5, 9.0 and 9.5 mg/l were tried
on L parsia fry of average weight 0.22 g, at 7 ppt salinity and at ambient
temperature 28 ± 2°C to determine the optimum dose of each. The optimum dose
was selected on the basis of behavioural pattern, induction and recovery times, ra.e
of oxygen consumption and post-treatment survival. The optimum doses obtained
for chloral hydrate, tertiary butyl alcohol and clove oil were 0 J 0 g/l, 2.5 mlll and 8.5
mg/l respectively. The effect of each anaesthetic at the selected dose on the
metabolic rate of the fry was compared, and significant difference was observed
amongst the three anaesthetics and control at these doses. Chloral hydrate and clove
oil treatment resulted in lower oxygen consumption rate than that of tertiary butyl
, '..11:' _ O;-=-, : ••• - '.' .- • ..c:'
alcohol treatment
No significant effect was observed on the percentage survival and duration
of the fry treated with the three anaesthetics at the selected doses in the oxygen-
packed jars. Packing density had significant effect on the sur vival and duration of
oxygen-packed fry. The safe duration with 100% survival was 15. ,6 h at 100 fry/I
and 3 to 7 hat 200, 300, 400 and 500 fry/I.
Linear relationships could be established in the case of (1) chloral hydrate
treatment, in the oxygen-packed jars at 70% survival between (a) packing density
and dissolved oxygen levels, (b) packing density and carbon dioxide levels and (c)
packing density and ammonia -N levels and (2) for tertiary butyl alcohol treatment

91
between (a) packiruz density and dissolved oxygen levels and (b) packing density and
carbon dioxide levels Significant difference was observed in the carbon dioxide
levels between treated and control jars, while no significant difference was noted in
the dissolved oxygen and ammonia -N levels in the treated and control jars

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