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    Chlorophyll content as an index for selection in Rauwolfia spp.
    (Kerala Agricultural University, 1996) Narayanan, A K; Luckins C Babu; Namboodiri, K M N; Achamma Oommen; Augustine, A
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    Isoenzyme variation and species relationship in genus piper
    (Kerala Agricultural University, 1996) Abraham Sebastian; Sujatha, V S; Nybe, E V; Sreekandan Nair, G; Augustine, A
    Eleven species of Piper including Piper rugrum were studied for variations in isoenzymes of three enzymes viz., peroxidase, esterase and glutaraate oxaloacetate transaminase. On grouping based on isoenzyme similarity, P. nigrum Linn.. P. pseudonigrum Velayudhan and Amalraj, P. bababudani Rahirnan and P. galeatum DC formed one group while P. argyrophyllum Miq. and P. attenuatum Buch-Hara constituted the second group and P. chaha Hunter, P. hapnium Miq. and P. colubrinum Link emerged as the third group. P. betle Linn, and P. longum Linn, showed their distinctness from the rest of the species. Least similarity was observed between P. colubrinum on one side and P. pseudonigrum and P. bahabudam on the other side.
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    In vitro production of toxic metabolite(s) by phytophthora capsic1 and partial purification of the metabolite(s)
    (Kerala Agricultural University, 1997) Shylaja, M R; Sreekandan Nair, G; Augustine, A; James Mathew
    Phytophthora capsici, the causal organism of Phytophthora foot rot disease in black pepper produces toxic metaholite(s) under in vitro conditions. Maximum accumulation of toxic metabolite(s) was observed in shake cultures of 15 days incubation in Ribeiro's medium. The symptoms induced by toxic metabolite(s) were quite typical to symptoms of natural and artificial infection by the pathogen. The toxic metabolite(s) accumulated in the in vitro culture was found to be heat stable aim non-specific. The toxic metabolite(s) could not be separated using organic solvent fractionation since it is present in the aqueous fraction of the culture filtrate. However, ion exchangers like Dowex 1 and Dowex 50 could be used for separating the metabolite(s) from the aqueous fraction.