2. Institutional Publications
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Item Screening of black pepper (Piper nigrum L.) calliclones for phytophthora foot rot resistance / tolerance(Kerala Agricultural University, 1996) Shylaja, M R; Sreekandan Nair, G; James MathewCalliclones of different black pepper cultivars viz., Kalluvally, Cheriakanyakkadan, Balankotta, Karimunda and Panniyur-1 were regenerated from calli, screened against toxic metabolite(s) of Phytophthora capsici. The regenerants derived from screened and unscreened calli were further tested for resistance / tolerance to P. capsici using different methods of screening viz., natural screening (keeping in infected field), screening by electrolyte leakage method and screening by artificial inoculation with culture disc of P. capsici. None of the regenerated calliclones was found to be completely resistant to the disease in natural screening. However, some of the regenerants derived from unscreened calli exhibited higher degree of tolerance to the disease revealing the possibility of exploiting somaclonal variation for Phytophthora foot rot disease screening in black pepper. The calliclones of Cheriakanyakkadan recorded greater degree of tolerance to the disease as compared to others.Item Effect of toxic metabolite(s) of phytophthora capsici on various stages of morphogenesis of black pepper calli(Kerala Agricultural University, 1997) Shylaja, M R; Sreekandan Nair, GThe effect of toxic metaholite(s) of Phytophthora capsici on various stages of morphogenesis of black pepper calli was studied. The survival rate of the call! in toxin medium was influenced by the varieties / cultivars used for the study. Once the calli survived in the toxin medium, the toxic tnetabolite(s) did not inhibit further growth of the calli. The shoot proliferation and elongation were also not influenced by the metabolite(s) in the media. However, the root growth was affected adversely.Item In vitro production of toxic metabolite(s) by phytophthora capsic1 and partial purification of the metabolite(s)(Kerala Agricultural University, 1997) Shylaja, M R; Sreekandan Nair, G; Augustine, A; James MathewPhytophthora capsici, the causal organism of Phytophthora foot rot disease in black pepper produces toxic metaholite(s) under in vitro conditions. Maximum accumulation of toxic metabolite(s) was observed in shake cultures of 15 days incubation in Ribeiro's medium. The symptoms induced by toxic metabolite(s) were quite typical to symptoms of natural and artificial infection by the pathogen. The toxic metabolite(s) accumulated in the in vitro culture was found to be heat stable aim non-specific. The toxic metabolite(s) could not be separated using organic solvent fractionation since it is present in the aqueous fraction of the culture filtrate. However, ion exchangers like Dowex 1 and Dowex 50 could be used for separating the metabolite(s) from the aqueous fraction.Item Response of black pepper (Piper nigrum L.) variety panniyur-1 and cultivars to indirect organogenesis(Kerala Agricultural University, 2000) Shylaja, M R; Sreekandan Nair , GIndirect organogensis was attempted in the black pepper variety Panmyur-1 and four cultivars viz. Karimunda, Kalluvally, Balankotta and Cheriakanyakkadan from expianls of axenic seedlings. The response of variety/cultivars at various stages of morphogenesis was studied in detail. The response suggests the manipulation of media specifically to each variety / cultivar to achieve the maximum morphogcnelic potential.