2. Institutional Publications
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Item Response of cowpea Vigna unguiculata (L.) Walp. to Rhizobium seed inoculation(Kerala Agricultural University, 1980) Sivaprasad, P; Shivappashetty, KField experiment was carried out at the Instructional Farm of the University of Agricultural Sciences, Bangalore to study the response of cowpea to seed inoculation with selected Rhizobium strains. Significant increase in yield, plant top dry weight and leghaemoglobin content of nodules were noticed in plants inoculated with the strains 8 and 12, Correlation studies showed significant correlation between leghaemoglobin content of nodules, plant top dry weight and final grain yield. No significant correlation was observed between nodule number and nitrogen content of plant top on Thirtieth day of growth and final grain yield.Item Occurrence of endotropic mycorrhiza in rubber (Heavea brasiliensis muell. Arc.)(Kerala Agricultural University, 1982) Sivaprasad, P; Rajendran Pillai, M V; Nair, M CItem Mechanism of enhanced nodulation in vesicular-arbuscular mycorrhizal (Vam) pigeonpea (Cajanus cajan (L.) Mill sp.)(Kerala Agricultural University, 1987) Sivaprasad, P; Rai, P VNodule and root initiation in Cajanus cajan plants inoculated with vesicular— arbuscular mycorrhizal fungus and Rhizobium was studied Mycorrhizal association enhanced the success of nodule formation with no significant difference in number of root initiated in a specific area. Further, there was increased cytokinin activity in stem exudate and root of mycorrhizal plants. Based on the observations a hypothesisis proposed for the mechanism of enhanced nodulation in mycorrhizal legumes.Item Ultra structures of nodules of cajanus cajan (L) mill sp. Inoculated with vesicular-arbuscular mycorrhizal (Vam) fungus(Kerala Agricultural University, 1988) Sivaprasad, P; Rai, V; Joshi, S SUltrastructural observations made on nodules of Cajanus cajan (L) Millsp. indicated that tha association of VA mycorrhizal fungus Glomus fasciculatum did not influence tha number of bacteroid formed per membrane envelop, which was always one. Both the transformed cell size and number of bacteroid/ Rhizobium pressnt in transformed cell were increased due to mycorrhizal association. Bacteroidal shape differed from the Rhizobium However, no such difference was noticed in size.Item Histochemical changes in nodules of Cajanus cajan (L.) Mill sp. inoculated with vesicular- Arbuscular Mycorrhizal Fungus and Rhizobium(Kerala Agricultural University, 1988) Sivaprasad, P; Joshi, S S; Rai, P VSymbiosis of Glomus fasciculatum (Thaxter sensu Gerd) and Trappe, Rhizobium and Ca/anus cajan (L.) Millsp. was studied in relation with bacteroidal zone and polysaccharide accumulation in nodule tissue. Tripartite symbiosis enhanced the area of bacteroidal zone in nodule tissue and size of bacteroidal cell and nucleus. Association of vesicular-arbuscular rnycorrhizal fungus alone had maximum polysaccharide accumulation in the nodule tissue. Dual inoculation with rnicrosymbionts showed very little polysaccharide accumulationItem Hardening and ex vitro establishment of rose plantlets(Kerala Agricultural University, 1997) Wilson; Nayar, N K; Sivaprasad, PPlantlets regenerated by in \ilro techniques under high humidity and low temperature in an artificial medium did not survive by direct planting out in small pots. An improvised structure made of wooden frame and polyethylene cover helped to maintain high humidity to ex vitro plantlets. Attempts made to get successful field establishment with different potting media and nutrient solutions did not give any positive results. Successful hardening and ex vitro establishment of plantlets were achieved by surface inoculation of different species of vesicular arbuscular mycorrhizae {VAM). Inoculation with Glomus etunicatum (Becker and Gerd) recorded the highest survival rate of 66.67 per cent, and took minimum days for flowering.