2. Institutional Publications

Permanent URI for this communityhttp://localhost:4000/handle/123456789/96

Browse

Author: search.filters.author.Sreekandan Nair, GSubject: search.filters.subject.Phytophthora foot rot

Search Results

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Screening of black pepper (Piper nigrum L.) calliclones for phytophthora foot rot resistance / tolerance
    (Kerala Agricultural University, 1996) Shylaja, M R; Sreekandan Nair, G; James Mathew
    Calliclones of different black pepper cultivars viz., Kalluvally, Cheriakanyakkadan, Balankotta, Karimunda and Panniyur-1 were regenerated from calli, screened against toxic metabolite(s) of Phytophthora capsici. The regenerants derived from screened and unscreened calli were further tested for resistance / tolerance to P. capsici using different methods of screening viz., natural screening (keeping in infected field), screening by electrolyte leakage method and screening by artificial inoculation with culture disc of P. capsici. None of the regenerated calliclones was found to be completely resistant to the disease in natural screening. However, some of the regenerants derived from unscreened calli exhibited higher degree of tolerance to the disease revealing the possibility of exploiting somaclonal variation for Phytophthora foot rot disease screening in black pepper. The calliclones of Cheriakanyakkadan recorded greater degree of tolerance to the disease as compared to others.
  • No Thumbnail Available
    Item
    In vitro production of toxic metabolite(s) by phytophthora capsic1 and partial purification of the metabolite(s)
    (Kerala Agricultural University, 1997) Shylaja, M R; Sreekandan Nair, G; Augustine, A; James Mathew
    Phytophthora capsici, the causal organism of Phytophthora foot rot disease in black pepper produces toxic metaholite(s) under in vitro conditions. Maximum accumulation of toxic metabolite(s) was observed in shake cultures of 15 days incubation in Ribeiro's medium. The symptoms induced by toxic metabolite(s) were quite typical to symptoms of natural and artificial infection by the pathogen. The toxic metabolite(s) accumulated in the in vitro culture was found to be heat stable aim non-specific. The toxic metabolite(s) could not be separated using organic solvent fractionation since it is present in the aqueous fraction of the culture filtrate. However, ion exchangers like Dowex 1 and Dowex 50 could be used for separating the metabolite(s) from the aqueous fraction.