In vitro propagation of Rose cv. Folklore

Abstract

Surface sterilisation, stage of explant, media for culture establishment and multiple shoot induction were standardised for in vitro propagation of rose cv. Folklore. Treatment with mercuric chloride 0.08% for 12 min was found to be optimum for the surface sterilisation of axillary bud explants. Axillary buds excised four days after flower opening, having 1.0 cm length, exhibited the best response in culture establishment. The Murashige and Skoog (MS) basal medium supplemented with benzyl aminopurine (BAP) 2.5 mg I 1 + 2.4 dichlorophenoxy acetic acid (2,4-D) 0.5 mg 1' was found to be the suitable medium for culture establishment. The best combination for multiple shoot induction was MS basal medium supplemented with kinetin 2.0 mg 1' + gibberellic acid (GA,) 1.0 mg 1'.