In vitro plant regeneration from shoot tip culture of blackgram (Vigna mungo (L.) Hepper)

Abstract

In vitro culture of blackgram | Vigna mango (L.) Hepper] cv. LBG was initiated from 7 day old aseptic seedling shoot tips. Isolated shoot tips were cultured on MS medium containing various concentrations and combinations of cytokinins and auxins. High frequency of callusing and shoot differentiation was observed on MS medium supplemented with BAP (3.0 nig 1') and NAA (1.5 mg 1 '1. A single shoot tip produced more than 12 shoots. The regenerated shoots were rooted on MS medium supplemented with different concentrations of IBA. Seventy five per cent of the shoots produced r<x>ts, and 90-95% of them survived in the field condition