Seed set in ginger (Zingiber officinale rosc.) through in vitro pollination

Abstract

Different in vitro pollination techniques viz., stigmatic, srylar, intra-ovarian, placenta!, modified placental pollination and test-tube fertilization were tried in ginger flowers. The pollen grains suspended in ME, medium were used for pollination. The pollinated pistil or its part was cultured in the medium of 1/2 MS + NAA 0.5 mg 1' + BAP 2.5 mg 1 ' + coconut water 15 per cent v/v. Seed development was obtained in placental, modified placental pollination and test tube fertilization. The seed germinated under in vitro condition on supply of appropriate combination of 2,4-D, BAP and NAA