Crossability studies and analysis of incompatibility in three species of capsicum

Abstract

In the present study interspecific hybridization could be successfully done among 3 species of Capsicum viz., C. annuum, C. fruiteseens and C. pendutum by the technique of cross pollination after amputation of the upper part of style along with the stigma followed by application of 5% sucrose solution on cut surface of the style. Embiyological analysis of the fertilized ovaries of self and cross combinations showed that in the incompatible combinations the embryosacs degenerated indicating failure of fertilisation. Further when pollination was done on the cut surface of the amputated styles after the application of 5% sucrose solution normal embryo development could be observed as in the case of selfed ovaries.