Browsing by Author "Wilson"

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    Hardening and ex vitro establishment of rose plantlets
    (Kerala Agricultural University, 1997) Wilson; Nayar, N K; Sivaprasad, P
    Plantlets regenerated by in \ilro techniques under high humidity and low temperature in an artificial medium did not survive by direct planting out in small pots. An improvised structure made of wooden frame and polyethylene cover helped to maintain high humidity to ex vitro plantlets. Attempts made to get successful field establishment with different potting media and nutrient solutions did not give any positive results. Successful hardening and ex vitro establishment of plantlets were achieved by surface inoculation of different species of vesicular arbuscular mycorrhizae {VAM). Inoculation with Glomus etunicatum (Becker and Gerd) recorded the highest survival rate of 66.67 per cent, and took minimum days for flowering.
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    In vitro propagation of Rose cv. Folklore
    (Kerala Agricultural University, 1998) Wilson; Nayar, N K
    Surface sterilisation, stage of explant, media for culture establishment and multiple shoot induction were standardised for in vitro propagation of rose cv. Folklore. Treatment with mercuric chloride 0.08% for 12 min was found to be optimum for the surface sterilisation of axillary bud explants. Axillary buds excised four days after flower opening, having 1.0 cm length, exhibited the best response in culture establishment. The Murashige and Skoog (MS) basal medium supplemented with benzyl aminopurine (BAP) 2.5 mg I 1 + 2.4 dichlorophenoxy acetic acid (2,4-D) 0.5 mg 1' was found to be the suitable medium for culture establishment. The best combination for multiple shoot induction was MS basal medium supplemented with kinetin 2.0 mg 1' + gibberellic acid (GA,) 1.0 mg 1'.