PG Thesis

Permanent URI for this collectionhttp://localhost:4000/handle/123456789/2

Browse

Author: search.filters.author.James, P C

Search Results

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Survey on the incidence of salmonellae in meat animals
    (Department of Microbiology, College of Veterinary and Animal Sciences,Mannuthy, 1977) James, P C; Abdulla, P K
    Prior to this “Survey on the Incidence of Salmonellae in Meat Animals” the information on the serotypes of Salmonella prevalent and the magnitude of their occurrence in livestock in Kerala had been meagre except for the reports by Sulochana et al. (1973) and Balakrishna Pillai (1975). The work carried out during the present investigation has gathered more information on the prevalence of Salmonella in livestock in Kerala. In this study the prevalence of Salmonella serotypes in the different species of animals was probed. A total of 823 biomaterials, besides 50 drain samples were subjected to cultural screening. This venture resulted in the recovery of 56 strains of Salmonella. Serological identification of many of these strains proved the prevalence of S. typhimurium, S. weltevreden and S. urbana. The preponderatingly prevalent serotypes were found to be the former two. Pathogenicity studies employing S. typhimurium culture in mice, guinea pigs, rabbits, calves and piglets were conducted. All these animals were found to be susceptible to infection by S. typhimurium evincing varying degrees of clinical manifestations. The advantages of employing modified McConkey’s medium (Sharma, 1961) containing Mannitol instead of lactose and composite medium 1 and 11 developed by Chitin is et al. (1972) to differentiate Salmonella at primary screening level have been discussed.
  • No Thumbnail Available
    Item
    Secretory immunoglobulins of the duck (Anas platyrrhynchos domesticus)
    (Department of Microbiology, College of Veterinary and Animal Sciences, Mannuthy, 1996) Hari Kumar, A V; James, P C
    The profile and functional properties of the immunoglobulins of serum, bile, mucos of trachea and intestine of ducks were studied. The immunoglobulins were separated by salting out using ammonium sulphate. The various fractions of immunoglobulines were further resolved by Sephadex G-200 gel filteration which gave two peaks for serum and tracheal immunoglobulins and a single peak each for bile and intestinal immunoglobulins. The purity of these fractions were checked by immunodiffusion and immunoelectrophoresis. The different fractions obtained were quantified by single radial immunodiffusion. The level of fraction 1 in the bile ranged between 1718 µg/ml and 1959 µg/ml and that of serum, 1718.06 µg/ml to 2442 µg/ml, in the S.typhimurium treated groups. The level of fraction 1 in the NDV treated groups ranged from 1115 µg/ml to 1597.35 µg/ml in bile, and 1597.35 µg/ml to 1959.34 µg/ml in serum. The level of fraction 2 in serum ranged from 1797 µg/ml to 2591 µg/ml in S.typhimurium treated group and 1400 µg/ml to 1797 µg/ml in the NDV treated group. Fraction 2 was not detectable in bile. The antibody response of ducks to a bacterial and viral antigen (anaculture of S.typhimurium and R2B strain of New Castle Disease virus respectively) was assessed. On conducting standard tube agglutination test, the serum, bile and oviduct washings revealed antibody tires against S.typhimurium in inoculated birds ranging between 1:20 and 1:160 in the case of serum; 1:10 and 1:80 in the case of bile and tire less than 1:10 for oviduct washings. No antibody titre could be detected for tracheal and intestinal washings and testicular extracts. The HI titre ranging from 1:32 to 1:128 could be observed for serum, 1:32 to 1:64 for bile and a titre of 1:16 was observed for oviduct washings of ducks parenterally administered with NDV. The tire was relatively low for serum when NDV was administered intranasaly. Intestinal and tracheal washings and testicular extract failed to reveal any HI antibodies.