PG Thesis

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    Analysis of inbreeding depression in west coast tall coconut (Cocos nucifera L.)
    (Department of Plant Breeding and Genetics, College of Agriculture, Padannakkad, 2016) Chethana, S; Jayaprakash Naik, B
    In nature, coconut is a cross pollinated crop which influences the high degree of variability and heterogeneous population. With the intension of developing inbred line in coconut, the programme was initiated in 1924 by selecting the eighteen WCT palms and developed S 1 generation. The S 1 plants were selfed and sibmated to produce the S 2 progenies in 1960 and the seedlings planted at CRS (RARS), in Pilicode with the replicated trial. This served the present experimental material to characterize and analyze inbreeding depression in S 2 palms and to study the effect of selfing (S 3 ) in selfed and sibmated (S 2 ) families of West Coast Tall (WCT). Vegetative, reproductive and nut characters were recorded in S 2 WCT family. The analysis of variance revealed significant differences among the twelve families of six groups of WCT for all the characters studied. The tallest palms were observed in IVS 2 -2 and IAS 2 -2 and on par with the WCT. The shortest palms were recorded from IIS 2 -1 and IIIS 2 -2 but taller than dwarfs. In general the sibmated families were showing more tall stature indicating heterotic behavior. The IAS 2 -1 and IBS 2 -1 showed average number of functional leaves as tall while IIIS 2 -1 had less number of leaves similar to dwarfs. The petiole length was short in IAS 2 -1 and IAS 2 -2 as well as IIIS 2 -1, IVS 2 -2 and VS 2 -1 indicating an advantageous character of high bearing of nuts with less loss. IIS 2 -1 and VS 2 -1 produced more number of female flowers while IAS 2 -1 and IAS 2 -2 produced less number of female flowers. In IIIS 2 -1 and VS 2 -1, the period of female phase similar to WCT. Thickest kernel was noticed in IBS 2 -1 and VS 2 -2 and less thick in IAS 2 -1. More thickness of kernel is tall palm character and thin meat is dwarf palm trait. Inbreeding depression was studied for S 2 nut yield and its attributes. The family IIS 2 -2 and VS 2 -1 expressed positive inbreeding depression for palm height and internodal length, but no inbreeding depression for stem girth. The leaf characteristics also exhibited positive inbreeding depression in S 2 . The WCTpalms in S 2 generation were characterized primarily based on eight characters separately when selfed and sibmated in S 1 for S 2 generation. In selfed the IBS 2 -1 showed tall characters with highest tall, stouter stem, medium functional leaves, and average female flowers, less setting percentage and low yield, medium nut, high oil percent. Semi tall characters in IIIS 2 -1 which recorded semi tall, stouter stem, average functional leaves, and average inflorescence produced, average female flowers, medium setting percentage, high copra and low oil content. Dwarf characters observed in IIS 2 -1 short, thin stem, high functional leaves, more inflorescence, high female flowers, medium setting percentage, nut yield, copra and oil content high. The S 3 seed nut showed that germination percentage was low in all the families (less than 50%) indicating the operation of deleterious effect of inbreeding in S 3 seed nuts. The semi-tall seedlings were more in selfed and talls in sibmated families of S 3 . The percentage of occurrence of stouter collar girth was more in S 3 (IAS 3 -1, IBS 3 -1, IIS 3 -1, IVS 3 -2, IIS 3 -2, IIIS 3 -2 and VS 3 -2). The leaf production was moderate in all the S 3 seedlings of selfed and four sibmated families (IAS 3 -2, IBS 3 -2, IIIS 3 -2 and VS 3 -2). Both the third and fifth leaves , the length, breadth and petiole length showed moderate values. The germination percentage revealed positive inbreeding depression in all the selfed families of 12 families. High inbreeding depression was noticed in IAS 3 -2, IIIS 3 -2, IVS 3 -2, VS 3 -1 and IVS 3 -1 and the lowest in family 1BS 3 -1 and 1AS 3 -1. Seedling height expressed high inbreeding depression in VS 3 -1. Collar girth didn‟t show inbreeding depression in S 3 . It is an important trait correlated with yield. Both positive and negative inbreeding depression was recorded for total number of leaves produced by the seedlings in S 3 generation. The molecular study using the promising inbreds was done using 10 primers. The analysis by the primers signifies that the family IIIS 3 -1 was unique which was noticed by the 5 primers i.e. OPBA 03, OPAW 19, OPAW 15, OPAW 09 and OPAU 02. The specific bands for dwarfs were noticed from almost all theprimers except for the OPAW 09. While the locus specific to tall have noticed from the primer OPBA 03 and OPAU 03. The specificity to the samples has also been noticed from some primers which was the WCT specific (OPAU 03), CGD (OPAW 15 and OPAW 08), IAS 3 -1 (OPAW 12, OPAW 13 and OPAW 08), IBS 3 - 1 (OPAW 19 and OPAU 03) and IIIS 3 -1 (OPAW 19). The coloured types IAS 3 -1 and IBS 3 -1 were highest and observed as on par with the WCT which were evidently proved by the primer OPBA 03 and OPAW 19. Among the primers, highly significant bands between the tall and dwarf was noticed by the OPBA 03 and OPAU 03. It is evident from the studies that the selfing of WCT palms would reduce vigour and possible to develop inbred lines which could be used for the production of hybrids for higher productivity.
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    Combining ability in bhindi (Abelmoschus esculentus (L) Moench)
    (Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 1995) Rajani, B; Manju, P
    The study was carried out in the Department of Plant Breeding and Genetics, College of Agriculture , Vellayani during 1994-95 to estimate the combining ability of six genetically divergent parental strains of bhindi and their 30 F1 hybrids obtained by crossing the six parents in a diallel pattern. The magnitude of heterosis and nature of gene action governing the yield of fruits and other important yield attributes was also elicited through Hayman's numerical and graphical approach. The combinig ability analysis by the Method 1 of Griffing's (1956) approach revealed that the parent P4 (NBPGR/TCR 864) was the best general combiner for yield and a few yield related characters. Among the hybrids P1 X P4 (NBPGR/TCR 893 x NBPGR/TCR 864) exhibited outstanding s.c.a effects for yield. Non addictive gene action was found to govern days to first flowering number of flowers per plant, length of fruit, girth of fruit, weight of single fruit, weight of fruits per plant and number of seeds per fruit while the remaining characters were governed by addictive gene action.
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    Cytomorphological and chemical studies on intervarietal crosses of Capsicum annuum, L.
    (Division of Agricultural Botany, Agricultural College & Research Institute, Vellayani, 1970) Manikantan Nair, P; Mary K George
    1. The cytomorphological and chemical aspects of four F1 hybrids of crosses involving five varieties of Capsicum annuum L. were studied . The variety ' Local blue' which possessed mosaic resistance , prolific bearing habit , long life span and high pungency was selected as the common seed parent. The selected pollen parents were, Russian, Indian long red , Chinese giant and Oskosh which were gifted with a higher content of ascorbic acid and sucrose and larger fruit size , but lacking the qualities of the common seed parent. 2. All the four F1 hybrids manifested a marked degree of heterosis in many economically important attributes like , carliness in blooming, number of leaves, number of branches, leaf area and chemical constituents like ascorbic acid and sucrose. 3. An intermediate condition was observed with regard to height , spread, number of fruits, fruit size and number of F2 seeds
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    Heterosis in snakegourd (Trichosanthes anguina L.)
    (Department of Olericulture, College of Horticulture, Vellanikkara, 1991) Philip Varghese; Rajan, S
    The present investigation "Heterosis in snakegourd" was conducted at the College of Horticulture during 1988-1990. Assessment of genetic variability showed significant differences of 25 characters in 48 snakegourd genotypes. The highest phenotypic coefficient of variation was observed for fruiting nodes on main vine, male flowers/plant, sex ratio and fruits/plant. The pcv was lowest for total crop duration. The gcv resulting in high heritability was high for majority of the characters. High heritability coupled with high genetic gain was noticed for male flowers/plant, sex ratio and fruiting nodes on main vine. The 48 genotypes were grouped into 10 clusters. The combing ability analysis revealed significant gca variances for all characters. The sca variances were also significant for all characters. The sca variances were also significant for all characters except for total crop duration, sex ratio and fruits/plant.
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    Evaluation of the productivity of chilli hybrids
    (Department of Plant Breeding, College of Agriculture, Vellayani, 1986) Elizabeth Philipose; Manikantan Nair, P
    A study was conducted at College of Agriculture, Vellayani, for evaluating the productivity of six intervarietal chilli (Capsicum annuum L.) hybrids obtained by crossing four inbreds in all possible combinations without reciprocals. Since the germination of the combination PR x PCl was poor, the other five hybrids along with four parents were evaluated in a 9 x 3 RED during 1984-85 and 1985-86. Observations on twenty quantitative and five five qualitative attributes were recorded. The statistical analysis included analysis of variance , pooled analysis and the estimation of genetic parameters, correlations among sixteen quantitative traits and three types of heterosis namely relative heterosis, heterobeltiosis and standard heterosis
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    Generation mean analysis in yard long bean (Vigna unguiculata subsp. sesquipedalis (L.) Verdcourt) for yield and quality
    (Department of Vegetable Science, College of Agriculture, Vellayani, 2018) Merin Elza George; Sarada, S
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    Breeding for shattering resistance in rice (Oryza sativa L.)
    (Department of Plant Breeding and Genetics, College of Horticulture, Vellanikkara, 2018) Anju M Job; Biju, S
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    Cause-consequence analysis of human-wildlife conflict at Nemmara forest division, Kerala
    (Department of Forest Management and Utilization, College of Forestry, Vellanikkara, 2017) Anand, R; Gopakumar, S
    A study titled “Cause-consequence analysis of human-wildlife conflict in Nemmara forest division, Kerala” was conducted during 2013-2017. The main objectives of the study were to portray the nature and distribution of human-wildlife conflict, study the land use and land cover change pattern. Another objective of the study was to assess the attitude and awareness of the people including the victims of human-wildlife conflict about forest conservation and human- wildlife conflict. The observations of the study would be made useful to evolve location specific management strategies at Nemmara Forest Division. A detailed pre tested questionnaire was used to gather information regarding socio-demographic details, land use and land cover change patterns, human wildlife conflict data, crop farming details and also attitude and awareness of the people. A total of 398 individuals were surveyed from Alathur, Kollengode and Nelliyampathy ranges of the forest division. Participatory Rural Appraisal (PRA) methods such as oral history, time line and problem tree methods were used to collect additional information. The study found out that major conflict areas were along the fringes of the forest areas. The type of conflict even though was mainly crop raiding, the nature of damage and the animal causing the damage were found to be different in different ranges in the forest division. Over the past 30 years there was significant change in the land use and land cover in the division. Over all, cropping pattern has changed from food crops to cash crops. The number of wild animals has increased and this was portrayed as the main reason for human-wildlife conflict. The people found the forest department as the best responsible agency to look up to take action regarding conflict mitigation and were also ready to join hands with them to resolve such issues. Even though the people had a positive attitude towards forest conservation and wildlife they were not satisfied with the conservation policies and human-wildlife conflict mitigation schemes of the government. The people did not favour compensation or insurance schemes as a mitigation strategy towards crop raiding mainly because of the low compensation amounts and difficulty in applying for such schemes. Site specific plans, based on the data generated from this study can be used to develop not only location specific plans but also both animal as well as people specific plans for conflict management and mitigation.
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    Characterization of okra [Abelmoschus esculentus L. Moench] genotypes in North Kerala
    (Department of Plant Breeding and Genetics, College of Agriculture, Padannakkad, 2017) Aswathi G Prasad; Namboodiri Raji Vasudevan
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    Variability in inter varietal hybrids and open pollinated seed progenies of black pepper ( Piper nigrum L .)
    (Department of Agricultural Botany, College of Horticulture, Vellanikkara, 1991) Sujatha, R; Narayanan Namboodiri, K M