1. KAUTIR (Kerala Agricultural University Theses Information and Retrieval)

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    Shelf life extension of nendran banana (Musa. spp.) using aloe gel incorporated silver nanoparticles
    (Department Postharvest Management, College of Agriculture,Vellayani, 2024-03-02) Athira Preman, M.; Athulya, S Kumar
    The study entitled “Shelf life extension of Nendran banana (Musa spp.) using aloe gel incorporated silver nanoparticles” was conducted at Department of Postharvest Management, College of Agriculture, Vellayani during 2021-2023, with the objective of standardizing aloe gel incorporated silver nanoparticles for shelf life extension of Nendran banana. The experiment was conducted as three parts. In the first part, plant mediated synthesis of silver nanoparticles (Ag NPs) was done and characterized for its size, shape and properties using Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Particle Size Analyser (PSA) and Raman Spectroscopy. In the second part, freshly harvested good quality Nendran banana bunches of commercial maturity were collected from Instructional Farm, College of Agriculture, Vellayani, de-handed fruits were ozonized (2 ppm) and subjected to different postharvest treatments in various concentrations of aloe gel incorporated silver nanoparticles (20% aloe gel with 40 ppm, 60 ppm, 80 ppm Ag NPs; 30% aloe gel with 40 ppm, 60 ppm, 80 ppm Ag NPs; 40% aloe gel with 40 ppm, 60 ppm, 80 ppm Ag NPs; 50% aloe gel with 40 ppm, 60 ppm, 80 ppm Ag NPs), aloe gel (20%, 30%, 40%, 50%) by dipping for 5 minutes along with absolute control (without any treatment). The treated banana fruits were air dried and stored under ambient temperature in Corrugated Fibre Board (CFB) boxes till the end of shelf life. The effectiveness of treatments was analysed based on physiological, biochemical and sensory parameters. Nendran banana fruits coated with 20% aloe gel incorporated with 40 ppm, 60 ppm, 80 ppm Ag NPs and fruits with 20% aloe gel coating exhibited maximum shelf life (14 days) with minimum physiological loss in weight and superior biochemical and sensory parameters, whereas the fruits without any treatment (absolute control) had a shelf life of 9 days. These four superior treatments were selected for further storage studies in the third part of the experiment. Nendran banana fruits were harvested at commercial maturity, sanitized (2 ppm ozone) and subjected to the four superior postharvest treatments. The treated fruits packaged in CFB boxes, were kept under cold storage (13± 2ºC) and low temperature conditions (below 10ºC) along with untreated fruits (absolute control) till the end of shelf life. Nendran banana fruits coated with 20% aloe gel incorporated with 80 ppm Ag NPs recorded maximum shelf life (34.25 days) with minimum physiological loss in weight (10.19%) under cold storage condition. When biochemical parameters were analysed, fruits coated with 20% aloe gel incorporated with 80 ppm Ag NPs recorded the minimum moisture content (61.70%), Total Soluble Solids (14.47°brix), reducing sugar (13.84%) and total sugar (14.44%) were recorded after 32 days of storage. They also exhibited maximum antioxidant activity (76.91%), ascorbic acid content (13.56 mg 100 g-1) with superior mean score for sensory parameters at the end of shelf life. The fruits without any treatment had a shelf life of 27.50 days with high physiological loss in weight (12.20%). Nendran banana fruits stored under low temperature condition exhibited chilling injury and hence discarded after 3 days of storage. The postharvest treatments extended the shelf life of Nendran banana fruits by delaying ripening and reducing physiological activities. Postharvest treatment of commercial maturity Nendran banana hands with 20% aloe gel incorporated with 80 ppm Ag NPs could give a shelf life of 34.25 days with superior physiological, biochemical and sensory quality parameters when stored in CFB boxes under cold storage (13± 2ºC) compared to 27.5 days for untreated fruits.