1. KAUTIR (Kerala Agricultural University Theses Information and Retrieval)
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Item Quality and storage life of oriental pickling melon [Cucumis melo var.conomon(L) Makino] as influenced by major nutrients(Department of Olericulture, College of Horticulture, Vellanikkara, 1985) Lissamma Joseph; Ramachandran Nair, SThe oriental pickling melon (Cucumis melo var. cocomon (L) Makino) was grown in a randomised block design under different organic and inorganic fertiliser treatments, with four replications at the Main Campus of Kerala Agricultural University, Vellanikkara, to study the effect of organic and inorganic manures on the yield, quality and storage life of this crop. The yield response of oriental pickling melon to different treatments was found to be significantly different. The treatment which received the highest dose of NPK in the organic and inorganic combination recorded the maximum yield per plot and number of fruits per plot whereas the treatments which received NPK through organic manures alone recorded relatively low yield. The weight and size of fruits were maximum when the highest dose of NPK was given completely in the inorganic form. The quality of the fruit was assessed by estimating the nutrient content at harvest; moisture, acidity, vitamin C, starch, total soluble solids and crude fibre content of fruits at harvest and thereafter at monthly intervals during storage. Significant difference was observed between treatments for these quality characteristics. During storage moisture, starch and vitamin C content of fruits showed steady decline irrespective of the treatments, whereas acidity and total soluble solids showed an initial increase followed by a gradual decline. The effect of organic and inorganic manures on storage life of oriental pickling melon was assessed by recording the rotting percentage of fruits during storage and it was found that increased degree of rotting occurred in treatments which received inorganic form of NPK, during all periods of storage. The organic form of manures showed a definite advantage over inorganic fertilisers in respect of the storability of oriental pickling melon.Item Genetic transformation of black pepper (piper nigrum L) for phytophthora foot rot resistance/tolerance(Department of Plantation Crops and Spices, College of horticulture, Vellanikkara, 2007) Lissamma Joseph; Valsala, P AInvestigations on "Genetic transformation of black pepper (Piper nigrum L.) for Phytophthora foot rot resistance/tolerance" were carried out at the Department of Plantation Crops and Spices, and Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Vellanikkara during 2001-2006. Three selected black pepper varieties Panniyur 1, 4 and 6 were utilized for the study. Axenic cultures of the selected varieties were raised from nodal segments as well as ripe seeds for embryogenesis and transformation studies. Among the varieties average number of multiple shoot/explant was high for variety P6 in ½ MS medium with BA and IAA 1.0 mg l-1. Somatic embryogenesis was induced on ripe seeds of the variety P6 at the micropylar region in ½ MS medium with inositol 1.0 g l-1. Further development of the somatic embryo observed in liquid shaking cultures of SH basal. Growth regulators like BA,2,4-D ,dicamba and thidiazuron did not give rise to embryogenesis in the different explants of black pepper. Multiple shoot induction from cotyledonary nodal explants and zygotic embryo explants were observed in all the varieties in ½ MS with 1.0 mg l-1 BA and IAA. Direct regeneration from leaf segments was also observed in the same media combination. Agrobacterium tumefaciens strains EHA105, AGL.1.1303, GV2260 and LBA4404 were used for the transformation work. Strain EHA105 contains the plasmid p35SGUSINT with gus A gene and npt II gene. The AGL.1.1303 contains the plasmid harbouring antibiotic resistant selectable marker genes (npt II and hpt IV) and GUS and GFP reporter genes. The GV2260 contain the plasmid pGV2260 with osmotin gene and npt II gene. The LBA4404 contains the plasmid pBZ100 containing alfalfa glucanase gene, rice chitinase gene and npt II gene. Sensitivity studies of black pepper tissues to various antibiotics resulted in selecting the optimum threshold level of antibiotic to be used in the screening medium. Kanamycin 25 mg l-1, 50 mg l-1, and 100 mg l- were selected as the cut off level for the selection of transformants from zygotic embryo, leaf segments and nodal segments respectively. Cefotaxime at 250 mg l-1 was selected for the effective elimination of Agrobacterium after infection. Genetic transformation was standardized with Agrobacterium strain EHA105 using leaf disc and zygotic embryo explants. Tentative protocol for transformation in black pepper include Agrobacterium inoculum density 0.9, infection time 10 min and co-cultivation period of 48 h. Acetosyringone at 50-100 µM favoured transformation. Transient gus assay revealed faint blue staining on the infected leaf explants. Explants, leaf segment, cotyledonary node and zygotic embryo were used for transformation with Agrobacterium strains AGL.1.1303, GV2260 and LBA4404. There was explant specificity for the different Agrobacterium strains used. With LBA4404 zygotic embryo explants gave maximum survival in the screening medium containing 50 mg l-1 kanamycin and 250 mg-1 cefotaxime. Direct gene transfer using gene gun attempted with pBZ100 and cotyledonary node explants. Bombarded explants were found surviving in the screening medium with kanamycin for four months. However molecular analysis of selected transformants through PCR revealed that npt II gene integration has not happened in the tissues subjected to PCR analysis.