1. KAUTIR (Kerala Agricultural University Theses Information and Retrieval)
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Item Secondary and micronutrient management for enhancing soil health and productivity in upland rice(Department of Soil Science & Agricultural Chemistry, College of Agriculture, Vellayani, 2018) Shamsida, P; Rani, BAn experiment entitled " Secondary and micronutrient management for soil health and productivity in upland rice " was conducted with medium duration rice variety Uma, to investigate the effect of secondary and micronutrient application under organic and integrated nutrient management practices, on nutrient uptake, soil health and productivity of upland rice. The field experiment was carried out at farmer‟s field, Venganoor, Thiruvananthapuram, during Virippu, 2017-„18. The experiment was laid out in a randomized block design with three replications and ten treatments viz. absolute control (T1), KAU PoP + lime (T2), KAU PoP + dolomite (T3), KAU PoP for organic farming (T4), foliar application of micronutrient solution or KAU sampoorna multimix along with lime (T5 and T6), dolomite (T7 and T8) and organic farming treatment (T9 and T10). Foliar sprays of 0.5 % micronutrient solution (containing FeSO4.7H2O 0.1%, ZnSO4.7H2O 0.25%, borax 0.1%, MnSO4. H2O 0.025%, and CuSO4. 5H2O 0.025%) and 1 % KAU sampoorna multimix (containing Zn 7%, B 4.5%, Cu 0.5%, Fe 0.2%, Mn 0.2% and Mo 0.02%) were given during the critical growth stages viz. active tillering, panicle initiation and one week after flowering. The soil of the experimental site before the crop was very strongly acidic with normal EC, sandy clay loam in texture, medium in organic carbon and available N, high in available P and K, sufficient in available Fe, Mn, Cu, Zn, exchangeable Ca and Mg and deficient in available B. The results of the study revealed that foliar application of micronutrient solution or sampoorna multimix along with KAU PoP (dolomite or lime) significantly increased the total and productive tillers per m2 with T7 giving the highest value. Root length, dry weight and volume were highest for all the organic farming treatments. Foliar application of micronutrients produced significant increase in yield and yield attributes with T8 recording the highest value for panicle weight (2.41 g), length of panicle (23.43 cm), per cent filled grains (87.17 %) and thousand grain weight (23.53 g) resulting in the highest grain yield (4158 kg ha-1). T8 was on par with treatments T5, T6 and T7. Highest straw yield was recorded by T5 (4897 kg ha-1) which was on par with the other micronutrient applied treatments (T6 to T8) and KAU PoP receiving lime or dolomite alone. Application of liming materials alone or along with foliar micronutrients gave significantly higher contents of N, P and K in index leaf at panicle initiation stage with T6 giving the highest value for N and P and T7 for K. Treatment T2 gave the highest Ca content of index leaf, which was also on par with all the micronutrient applied treatments. Mg content was highest in the dolomite applied treatments and was on par with the lime applied ones. Fe, Mn, Cu, Zn and B contents in index leaf were also found to be higher for the treatment receiving foliar micronutrients along with KAU PoP (dolomite or lime), with the highest values recorded by T5 for Fe, T7 for Mn and Zn, T6 for Cu and T8 for B. Higher N, P and K content in straw and grain were observed for treatments T5 to T8 which were on par with KAU PoP (lime or dolomite). Ca content of straw was highest for T2 and was on par with all the treatments except T4 and T1. Highest grain Ca was observed for T5 which also gave significantly higher Mg content in grain and straw compared to absolute control and the organic treatments. Uptake of N, P, K, Ca, Mg, Fe, Mn, Cu, Zn and B by straw and grain was also found to be the highest for the treatments receiving micronutrients along with KAU PoP (lime or dolomite). Soil available N and K were on par for all the treatments except organic treatments and absolute control. Available P was on par for all the treatments except T1. The increase in organic carbon due to integrated nutrient management was not significant. Liming materials significantly increased the soil Ca level whereas soil Mg was increased by the application of dolomite. Integrated or organic management increased the soil Fe, Mn and Zn levels. In general organic treatments gave significantly lower values for Cu and B. Foliar application of micronutrients along with KAU PoP (dolomite or lime) was more economical, resulting in significantly higher B: C ratio with the highest value (1.43) given by T8. Based on the study, it can be concluded that foliar micronutrient application (sampoorna multimix or micronutrient solution) along with KAU PoP (lime or dolomite) significantly enhanced the growth, yield and nutrient uptake in upland rice.Item Soil and nutrient management for supperssion of fusarium wilt disease of yard long bean (vigna unguiculata subsp. sesquipedalis (L.) verdcourt)(Department of Soil Science & Agricultural Chemistry, College of Agriculture, Vellayani, 2018) Sooraj, R S; Biju JosephItem Molecular Characterization of sweet potato feathery mottle virus(Department of Plant Biotechnology, College of Agriculture, Vellayani, 2017) Ashna, N K; Makeshkumar, TA study on “Molecular characterization of Sweet potato feathery mottle virus” was conducted at the ICAR- Central Tuber Crop Research Institute, Sreekariyam, Thiruvananthapuram during 2016-2017. During the study 45 accessions of sweet potato from different fields of ICAR- CTCRI were selected and different symptoms of Sweet potato feathery mottle virus infection were recorded. The study reveals that the rate of infection is still prevailing in Kerala having high symptom severity due to combination with other potyviruses and increased aggressiveness as compared to earlier years because of the presence to different strains. Total RNA was isolated from all samples and used for RT-PCR analysis by degenerate primers for potyviruses and specific primers for SPFMV to differentially identify the presence of SPFMV. From the 45, five were confirmed as SPFMV positive. Among this amplified products obtained from sample SH710 was sequenced. From the 8 clones sequenced 3 different isolates of SPFMV is obtained, Sweet potato feathery mottle virus isolate SPFMV-O-Arg polyprotein gene (NCBI ID: KF386013.1) from Argentina, Sweet potato feathery mottle virus isolate CW137 (NCBI ID: KP115608.1) from Korean and Sweet potato feathery mottle virus isolate Shanxi2 coat protein gene (NCBI ID: HQ844123.1) from China, these are further belongs to broad class of Ordinary and Russet crack and East African. It further showed the possibility of presence of RC-strain O-strain and EA-strain in Kerala. To identify the phylogenetic relationship of the sequenced samples with that of available accessions, dendrograms were made using MEGA 7.0.26 software and the tree showed that sequences has variability eventhough lies within the group. By restriction analysis and sequence analysis, variability was recorded within SPFMV in Trivandrum.