1. KAUTIR (Kerala Agricultural University Theses Information and Retrieval)
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Item Standardization of tree injection procedures of Azadirachtin in coconut (Cocos nucifera L.), mango (Mangifera indica L.) and neem (Azadirachta indica A Juss.)(Department of Forest Products and Utilization, College of Forestry,Vellanikkara, 2019) Sarmishtra, V; Anoop, E VTree injection is a new technology that is employed to apply fungicides, nutrients and pesticides in large trees in order to avoid drifting of these chemicals and affecting non target organisms. It eliminates the wastage of chemicals to be applied in trees as the quantity used is little compared to other conventional methods of application. This study aimed at standardizing the tree injection procedures in Indian conditions. Stem wood of three species like coconut (Cocos nucifera L.), mango (Mangifera indica L.) and neem (Azadirachta indica A. Juss.) were used in the study along with their leaves after the application of azadirachtin through injection. The depth to which the tree injection can be applied was determined by studying the thickness of the conducting tissues in these species using the software Digimizer. Three size classes like 50-60cm, 60-70cm, 70-80cm were studied. Thickness of high density wood in coconut palm and sapwood in mango and neem does not vary with the change in size class. Average high density wood thickness in coconut palm was 3.5cm and was not more than 4.89cm. Thus a depth of 6 cm was fixed so as to ensure the delivery of chemicals into the most active part of the stem. In case of mango tree, the average sapwood thickness was 6.18cm and was never smaller than 3.27cm. Similarly, average sapwood thickness in neem was 4.38cm and was never smaller than 3.02cm. Thus 3 cm was the depth fixed to inject chemicals in mango and neem. Systemic insecticide, Azajet (50,000ppm) was used to inject the trees. Each tree was marked at a basal height of 20 cm from ground. Holes were made at an angle of 45º to make sure that there was no oozing out of chemicals. The EcoJect pump consisting of a canister, nozzle and a cylinder with compressed air between 100 and 150 PSI was used to inject the chemicals. Two canisters of 20 ml each (40ml/tree) were used to deliver the chemical into the tree trunk. Physiological parameters like photosynthesis, transpiration, leaf temperature and leaf moisture were analyzed using Infrared Gas Analyzer (LI-6400, Portable Synthesis System). Stomatal rate was studied by the replica method. A correlation analysis was conducted between the anatomical and physiological properties of the three tree species. The traces of azadirachtin in the leaves were determined by collecting the leaf samples during specific time intervals like 1hr, 2hr, 6hr, 2 days, 7 days, 14 days, 20 days, 28 days, 40 days and 55 days of tree injection by using High Performance Liquid Chromatography (HPLC). There were no correlations between the anatomical and physiological parameters. Azadirachtin traces were found only in coconut palm on the second day of injection with a peak area of 0.14µg/g. Other trees showed no sign of azadirachtin in their leaves. The traces of the bio pesticide did not last for a week as there was no further detection of azadirachtin in the samples collected after 7 days of injection.Item Evaluation of bacillus thuringiensis berliner for the management of rice leaf folders(Department of Agricultural Entomology, College of Horticulture,Vellanikara, 1998) Asha, R; Babu Philip, MThe study was conducted with the objective of survey and identification of the natural enemies of leaffolder and other lepidopterous pests of rice and to evaluate the efficacy of B. t. formulations against the leaffolders of rice. The survey was conducted at the Agricultural Research Station, Mannuthy and the Kerala State Agricultural Department Seed Farm, Mannuthy. The field trials to evaluate the efficacy of rice leaffolders were conducted at the Agricultural Research Station, Mannuthy during Kharif 1995 and Kharif 1996 . .. The present study revealed the occurrence of five viroses, two bacterioses and a nematode infection in the various lepidopterous pests of rice. Four Braconids, three Ichneumonids one Eulophid, one Chalcid and a Bethylid parasite were also recorded from various lepidopterous pests of rice. The B.t. formulations tested were found to be effective against the leaf folders on rice. The percentage of leaf damage during Kharif 1995 showed the B.t. formulations Biotox, BTT, Biolep to be the best treatments. With respect ~to the grain yield, BTT was the best treatment followed by Dipel, Biolep and Biotox. During Kharif 1996, Dipel followed by Biotox and monocrotophos were found to be the best in terms of percentage of leaf damage. Based on the yield during 1996, BTT was found to be the superior one followed by monocrotophos. B. t formulations were found to be safe to predatory spiders, other predators and paras i toids of folders. the leaf The combination of BTT and C.m. GV was able t.o reduce the leaf damage and data on larval mortality showed the leaf folder larvae to be susceptible to the combination of BTT and C.m. GV. The GV of C. medinalis was found to be highly host specific.Item Eco-friendly management of major pests of upland rice ecosystem(Department of Agricultural Entomology, College of Agriculture, Vellayani, 2018) Shobha, Y B; Suja, GItem Management of American serpentine leaf miner Liriomyza trifolil (burgess) dietars in tomato(Department of Agriculture Entomology, College of Agriculture, Vellayani, 2017) Mithra Mohan; Anitha, NItem Biotic agents for the management of American serpentine leaf miner, Liriomyza trifolii(Burgess) (Diptera:Agromyzidae)(Department of agricultural entomology, College of horticulture, Vellanikkara, 2014) Jyothi Sara Jacob; Maicykutty P MathewA study on “Biotic agents for the management of American serpentine leaf miner, Liriomyza trifolii (Burgess) (Diptera: Agromyzidae)” was carried out at the Department of Agricultural Entomology, College of Horticulture, K.A.U., Vellanikkara during 2011-2013 with the objectives of collection and identification of indigenous natural enemies and to assess the pathogenicity of the entomopathogens to explore the feasibility of utilizing them for its management. Surveys were conducted in the vegetable fields for the collection and identification of natural enemies associated with L. trifolii in three districts, namely, Thrissur, Ernakulam and Kottayam from January to March, 2011. The surveys revealed the occurrence of nine species of hymenopteran parasitoids. The per cent parasitism varied from 10.96 to 58.99 per cent among the crops surveyed. Three species of eulophids, namely, Cirrospilus acadius Narendran, C. brevicorpus Shafee & Rizvi and Aprostocetus sp. as well as the braconid, Toxares sp. are new reports for India. Among the parasitoids, Closterocerus spp. were the dominant group followed by Chrysonotomyia sp. All parasitoids were solitary, larval endoparasitoids except Toxares sp. which was larval-pupal in nature. One species each of small ants (Formicidae) and a dipteran fly (Dolichopodidae) were observed as predators on L. trifolii. In the study, no entomopathogens were observed from L. trifolii. Considering the level of pesticide consumption in vegetable crops that undermine the potential of insect parasitoids and also that no entomopathogens could be observed during the survey, it was decided to evaluate entomopathogenic nematodes (EPNs) as biocontrol agents against L. trifolii. Isolation of EPNs from 72 soil samples from Thrissur, Ernakulam and Kottayam districts yielded four isolates of Steinernema carpocapsae. Bioefficacy studies carried out on these four isolates along with Steinernema bicornutum and Heterorhabditis indica showed that S. carpocapsae Isolate - 1 had the lowest LC 50 , LC 90 and LT values indicating their higher effectiveness against the maggots of the pest. 50 Pot culture study conducted to compare the potential of S. carpocapsae Isolate - 1 with other treatments showed that azadirachtin 1 EC at 0.005% was the most effective causing 84.51 per cent mortality to the maggots of L. trifolii. This was followed by the foliar application of H. indica at 32 infective juveniles (IJs)/ maggot which caused 18.98 per cent mortality. Application of Beauveria bassiana at 1×10 7 spores/ ml was not effective. In the field evaluation, fipronil 5 SC at 0.002% was found to be the most effective treatment for controlling L. trifolii followed by azadirachtin 1 EC at 0.005%. Compatibility of the IJs of the S. carpocapsae Isolate - 1, S. bicornutum and H. indica was studied with ten commonly used insecticides in the laboratory by direct exposure method. Chlorantraniliprole 18.5 SC at 0.005% was found to be the most compatible insecticide with S. carpocapsae isolate - 1 causing only 0.17 per cent mortality to IJs at 72 hours after treatment (HAT). Quinalphos 25 EC at 0.05% and chlorpyriphos20 EC at 0.05% were highly incompatible, causing 96.17 and 92.87 per cent mortality of the nematodes. Dimethoate 30 EC at 0.04% was the most compatible insecticide with S. bicornutum and caused only 0.60 per cent mortality at 72 HAT and was followed by azadirachtin 1 EC at 0.005% with 0.78 per cent mortality to the IJs. Quinalphos 25 EC at 0.05% caused 99.93 per cent mortality at 72 HAT. Heterorhabditis indica was compatible with all insecticides except quinalphos 25 EC at 0.05% which was moderately toxic resulting in 39.6 per cent mortality. The virulence, pathogenicity and multiplication of the survived IJs were not affected by the insecticide treatments. Parasitoids and EPNs were observed as potential candidates for the management of L. trifolii. Hence future studies on the bio-ecology and mass production of dominant parasitoids and standardization of methods to improve the efficacy of EPNs are suggested for the successful control of L. trifolii in polyhouses as well as in the field.