1. KAUTIR (Kerala Agricultural University Theses Information and Retrieval)

Permanent URI for this communityhttp://localhost:4000/handle/123456789/1

Browse

Has files: YesSubject: search.filters.subject.Tissue culture

Search Results

Now showing 1 - 7 of 7
  • No Thumbnail Available
    Item
    Comparative evaluation of biotization for hardening of tissue culture (TC) Banana cv. Nendran
    (Department of Fruit Science, College of Agriculture, Padannakkad, 2025-06-02) Sandra, T
    The study entitled “Comparative evaluation of biotization for hardening of tissue culture (TC) banana cv. Nendran” was carried out at RARS, Pilicode and College of Agriculture Padannakkad during 2023 to 2024 to study identification of ideal stage of biotization and comparative evaluation of biotization agents in hardening of TC banana cv. Nendran.The study comprised four experiments: in vitro culture, primary hardening, secondary hardening, and combined evaluation of biotization agents during hardening. The in vitro rooting stage experiment carried out in completely randomised design with four treatments and five replications. The treatments were T1 (Piriformospora indica along with rooting medium), T2 (Phosphate Solubilizing Bacteria (PSB) along with rooting medium), T3 (Pseudomonas fluorescens (PF) along with rooting medium), and T4 (Control: rooting medium). Among these, T1 significantly enhanced early root initiation, shoot proliferation, and overall rooting efficiency. Plantlets treated with P. indica (T1) during in vitro rooting showed superior performance during primary hardening, achieving the highest survival rate (93.33%) and enhanced growth traits: plant height (10.33 cm), pseudostem girth (2.47 cm), leaf length (5.23 cm), leaf width (1.93 cm), leaf area (15.39 cm2 ), number of primary roots (2.67), root length (2.80 cm), number of secondary roots (18.33), shoot dry weight (0.02 g), chlorophyll content (0.56 mg g-1) and relative growth rate (0.044 mg g-1 d-1). Lower proline content (1.04 µmol g⁻¹ FW) indicated reduced stress, and improved uptake of N, P, and K and micronutrients confirmed its role in nutrient acquisition. During secondary hardening, both T1 (P. indica) and T3 (Pseudomonas fluorescens) showed 100% survival, but T1 outperformed T3 in all growth parameters, including plant height (13.17 cm), pseudostem girth (2.33 cm), leaf length (8.80 cm), leaf width (2.67 cm), number of leaves (5.33), leaf area (59.93 cm2), number of primary roots (3.67), root length (10.47 cm), number of secondary roots (75), root fresh weight (0.44 g), shoot fresh weight (1.52 g), shoot dry weight (0.10 g) and relative growth rate (0.037 mg g-1 d-1). T1 also exhibited the lowest proline content (0.71 µmol g⁻¹ FW), suggesting better stress tolerance. The primary hardening experiment carried out in completely randomised design with eight treatments and three replications. The treatments were T1 (P. indica), T2 (PSB), T3 (PF), T4 (PF + PSB), T5 (PF + P. indica), T6 (PSB + P. indica), T7 (PF + PSB + P. indica) and T8 (control). Biotization with P. indica (T1) produced the highest survival rate (100%) and demonstrated superior plant growth, including significant increases in plant height (5.70 cm), leaf length (6.97 cm), leaf width (2.33 cm), leaf area (12.48 cm2), root length (5.97 cm) root fresh weight (0.62 g), chlorophyll content (0.47 mg g-1), relative growth rate (0.047 mg g-1 d-1), macronutrient and micronutrient uptake (N, P, K, Fe and Cu). Proline accumulation (1.25 µ mol. g-1 FW) was lower in T1, suggesting improved stress tolerance. The secondary hardening experiment carried out in completely randomised design with eight treatments and three replications. The treatments were T1 (P. indica), T2 (PSB), T3 (PF), T4 (PF + PSB), T5 (PF + P. indica), T6 (PSB + P. indica), T7 (PF + PSB + P. indica) and T8 (control). During this experiment, T7 showed excellent results, with improved growth metrics such as plant height (15.20 cm), leaf length (8.90 cm), number of leaves (5.67), leaf area (45.88 cm2), root length (12.07 cm), number of secondary roots (111.67 ), root fresh weight (0.65 g), chlorophyll content (0.46 mg g 1), shoot fresh weight (1.20 g), shoot dry weight (0.08 g), macronutrient and micronutrient uptake (N, Fe, Cu, Zn). Combined evaluation study was laid out in completely randomised design with 11 treatments and two replications. The treatments were T1 (P. indica during in vitro and primary hardening), T2 (P. indica during in vitro and primary and secondary hardening), T3 (PSB during in vitro and primary hardening ), T4 (PSB during in vitro and primary and secondary hardening), T5 (PF during in vitro and primary hardening), T6 (PF during in vitro and primary and secondary hardening), T7 (PF during in vitro + PSB during primary and secondary hardening), T8 (PF during in vitro + P. indica during primary and secondary hardening), T9 (PSB during in vitro + P. indica during primary and secondary hardening ), T10 (PF during in vitro + PSB + P. indica during primary and secondary hardening) and T11 (Control). T2 exhibited superior growth and stress tolerance compared to other treatments. This was evidenced by enhanced plant height (14.83 cm), leaf length (9.40 cm), leaf width (3.13 cm), number of leaves (6.00), leaf area (69.29 cm²), number of primary roots (5.67), secondary roots (86.67), root fresh weight (0.72 g), shoot dry weight (0.11 g), chlorophyll concentration (0.26 mg g⁻¹), and relative growth rate (0.04 mg g⁻¹ d⁻¹). Notably, lower proline accumulation (0.81 µmol g⁻¹ FW) indicated reduced abiotic stress. T2 also showed much increased nutrient uptake, primarily of Phosphorus (0.50%), Potassium (0.07%), Iron (225.24 ppm), Copper (26.95 ppm), Manganese (135.33 ppm), Zinc (43.87 ppm), and Boron (18.61 ppm).
  • No Thumbnail Available
    Item
    Improvement of Anthurium andreanum Lind. in vitro
    (Department of Pomology and Floriculture, College of Horticulture, Vellanikkara, 1998) Mini Balachandran; Ramachandran Nair, S
  • No Thumbnail Available
    Item
    Effect of spacing and planting material on the growth, yield and active principle in Plumbago rosea L.
    (Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 1990) Subha, S; Sreekandan Nair, G
    Investigations on "Effect of spacing and planting material on the growth, yield and active principle in Plumbago rosea L." was conducted at the Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara during 1987-88, using factorial RBD design for exploiting this valuable medicinal plant with the following objectives: (1). To standardise the size of shoots as planting material for commercial cultivation. (2). To standardise the best spacing for better growth, yield and active principle. (3). To explore the possibility of growing Plumbago rosea L. as a commercial crop. (4). To explore the possibility of grooming this plant as an annual plant.
  • No Thumbnail Available
    Item
    Genotypic evaluation and in vitro multiplication of anthurium (Anthurium andreanum linden) hybrids
    (Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2019) Anand, S; Beena Thomas
  • No Thumbnail Available
    Item
    Seed germination and tissue culture studies in orchids
    (Division of Horticulture, University of Agricultural Sciences, Bangalore, 1982) Ramachandran Nair, S; Foja Singh
  • No Thumbnail Available
    Item
    Effect of gooseberry (Emblica officinalis) and Indian gall nut (Terminalia chebula) on the immune response in cockerels under induced heat and cold stress
    (Department of Veterinary Physiology, College of Veterinary and Animal Sciences, Mannuthy, 2006) Mejo, K R; Ramnath, V
    The study was conducted with an objective of finding the physiological and immunological variations that could be brought about with heat /cold stress in cockerels and the role of Gooseberry (GB) and Indian gallnut (IGN) supplementation as an antistress. Gramapriya cockerels of 1kg bw (3-4 months) were subjected to heat (40 ±1o C and relative humidity (RH) 60-70 percent) and cold stress (8 ±1o C and RH 40-50 per cent) each for 4h/day in a controlled environmental chamber (floor space 875 cm2 /bird) for a maximum of 10 days and the controls were reared randomly under ambient temperature of 30±1o C and RH 65 percent. GB+IGN supplementation was done @ 2.0 g/kg for 20 days (prior to and during the period of heat/cold stress). To a certain extend alternations in haematological parameters such as haemoglobin, packed cell volume, H/L ratio, biochemical parameters such as serum total protein, albumin, globulin, C-reactive protein, electrolytes like sodium and potassium, enzymes like lactate dehydrogenase and creatine kinase, cortisol, triiodothyronine (T3), thyroxine (T4) could be reversed by GB+IGN supplementation during heat stress. In the present study, the haemagglutinin (HA), hemolysin (HL), IgG and IgM titres and the spleenic antibody forming cells (plaque forming cells) and rosette forming cells (RFC) against known antigen were studied. The results indicated that GB+IGN supplementation not only maintained the preformed antibody titre but also improved the humoral immune response against a challenged antigen during the period of heat stress. In the present study, it was found that during cold stress, the GB+IGN supplementation could bring about an early tendency to restore the normal homeostasis of haematological, biochemical, and hormonal parameters. Cold stress resulted in a low profile of humoral immune response indicated by low anti-SRBC haemolysin (HA), Haemolysin (HL), IgG and IgM titres in untreated, CST cockerels when compared to GB+IGN treated counterparts, which showed better tires during cold stress. Similarly, treated cockerels exhibited more spleenic cells that produce antibodies against rat red blood cells. Thus, the immunopotentiative property of GB+IGN was reconfirmed and that the drug supplementation stimulated the humoral arm of immunity in cold stressed cockerels. Results of the present study indicated that combined supplementation of GB+IGN @ 2.0 g/kg bw in poultry could augment the humoral response during heat and cold stress
  • No Thumbnail Available
    Item
    In vitro propagation of dalbergia latifolia roxb. through tissue culture
    (Department of Forestry, College of Forestry, Vellanikkara, 1992) Khages Chandra Mahato; Vijayakumar, N K
    In investigations carried out in the College of Forestry, Vellanikkara during 1989 – 92, it was found that nodal segments of 1.5 cm length were ideal as the explants. Prophylactic spraying of the mother tree with the systemic fungicides Bavistin and the contact fungicide Dithane M-45 coupled with surface sterilization of explants with mercuric chloride 0.1 per cent for 12 minutes fully controlled contamination of the culture. Both woody plant medium (WPM) and Murashige and Skoog (MS) medium were found to be suitable for the primary culture establishment from the explants. While WPM supplemented with kinetin 1.0 ppm and IAA 0.1 ppm was most suitable for inducing healthy single shoots in about 80 per cent of the explants, MS along with BA 2.0 ppm or BA 0.25 ppm and CH 1000 mg 1-1 induced maximum number of multiple shoots (up to 25). Among the various media supplements tested, adenine sulphate was found to be capable of inducing multiple shoots and CH increased the rate of shoot multiplication. Coconut water did not show any beneficial effects. Liquid cultures with shaking at initial periods prolonged the life of the primary culture up to six months with continuous production of shoots. Continuous culture was developed using nodal segments of shoots derived from the primary cultures. The most suitable medium for this was found to be MS supplemented with kinetin and BA 0.5 ppm each. The best in vitro rooting was achieved by resorting to a pulse treatment of the shoots with IBA (1000 ppm) and culturing them in vermiculite + sand medium. Up to 100 per cent rooting could be achieved by this method. In vivo rooting was obtained by transferring the shoots after IBA treatment to vermiculite under high humidity conditions. Planting out and hardening of the in vitro rooted plantlets was carried out in soilrite. Up to 90 per cent survival could be achieved. The hardened plantlets were acclimatized in polythene bags with ordinary potting mixture and after 16 weeks they were field planted. The cost of production of one plantlet including hardening was worked out to Rs. 4.47.