1. KAUTIR (Kerala Agricultural University Theses Information and Retrieval)
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Item Seed treatment in cowpea (vigna unguiculata L.) for seed quality enhancement using botanicals(Department of Seed Science and Technology, College of Agriculture, 2024-03-27) Kottagorla Venkata Satya Sai Krishna.; Roshni VijayanThe present investigation entitled “Seed treatment in cowpea (Vigna unguiculata L.) for seed quality enhancement using botanicals” was carried out at the Regional Agricultural Research Station (RARS) Pattambi, Kerala. The study aimed to assess the effect of seed hardening with organic leaf extracts on filed performance, seedling quality parameters and nutritional attributes of cowpea during the storage period. This study consists of three experiments (Experiment -1) Effect of seed hardening with organic leaf extracts on field performance of cowpea. It was evaluated by using randomized block design (RBD) with nine treatments including control (5% leaf extracts of Calotropis, Papaya, Pongamia, Castor, Moringa, Vitex, Neem, Tulsi and Control) and 3 replications. Seeds were soaked in 5% leaf extracts for four hours and then shade dried to their original moisture content. Crop raised as per POP recommendation of KAU (2016). Observations were recorded on growth and yield parameters during crop growth stages. The results revealed that the performance of seeds treated with organic leaf extracts was better compared to the control. A significant effect was observed in seeds treated with Pongamia leaf extract had given the highest values for plant height (145.07 cm), number of branches per plant (10.27), number of clusters per plant (7.53), number of pods per plant (12.13), seed yield per plot (353.62 g), dry matter production at harvest (56.95 g), and nodule count per plant (17.78). Seeds treated with Calotropis leaf extract had given the highest values for, pod length (17.18 cm) and pod weight (10.63g). Similarly, neem leaf extract had given the highest values for pod girth (2.2 cm) and 100 seed weight (12.88 g). (Experiment-2) The effect of seed treatment with organic leaf extracts on the seedling quality parameters of cowpea was evaluated in a completely randomized design with three replications. Seed treatment was done as in the previous experiment and then shade-dried the seeds for two days, bringing the moisture content to less than 8 %, and packed in a 700-gauge polythene bag kept for storage in ambient conditions. Observations were recorded on seedling quality parameters at the start of the storage, 45 DAS and 3 months after storage. It was observed that seeds treated with organic leaf extracts had better performance compared to control. A significant effect was observed in seeds treated with Pongamia leaf extract had given the highest values for emergence per cent (61.67 %), root length (10.33 cm), seedling dry weight (0.048 g), low electrical conductivity of seed leachate (0.467 dSm -1), emergence index (21.38) and seedling vigour index-II (3.03). Seeds treated with Calotropis leaf extract had given the highest values for germination per cent (63.67 %), low pulse beetle infestation (16.33 %), germination index (324), mean germination time (3.87 days), time taken for 50 % germination (3.34 days) and seedling vigour index-I (2259) by the end of the storage period. Seeds treated with Papaya leaf extract had given the highest values for shoot length (25.65 cm). Similarly, low seed microflora per cent (19.05%), was recorded in seeds treated with neem leaf extract by the end of the storage period. (Experiment-3) The effect of seed treatment with organic leaf extracts on the nutritional attributes of cowpea. Experiments 3 was carried out simultaneously with Experiment 2 by using the same seeds kept for storage in the Experiment 2. Observations were recorded on nutritional attributes which include crude protein, crude fat, crude fibre, vitamin C, calcium content, iron content, ash content, carbohydrate content and phytic acid content at the start of storage, 45 DAS and 90 DAS . A significant effect was observed in seeds treated with Pongamia leaf extract, which had given the highest values for calcium content (48.03 mg/100 g), iron content (2.42 mg/100 g), ash content (4.07 %) and carbohydrate content (51.03 %) by the end of the storage period. Among all the treatments, seeds treated with Pongamia leaf extracts had given the better performance compared to control in field performance as well as improvement in seedling quality parameters and preserving the nutritional attributes of cowpea during the storage period.Item Seed treatment in cowpea (vigna unguiculata L.) for seed quality enhancement using botanicals(Department of Seed Science and Technology, College of Agriculture, Vellanikkara, 2024-03-27) Kottagorla Venkata Satya Sai Krishna.The present investigation entitled “Seed treatment in cowpea (Vigna unguiculata L.) for seed quality enhancement using botanicals” was carried out at the Regional Agricultural Research Station (RARS) Pattambi, Kerala. The study aimed to assess the effect of seed hardening with organic leaf extracts on filed performance, seedling quality parameters and nutritional attributes of cowpea during the storage period. This study consists of three experiments (Experiment -1) Effect of seed hardening with organic leaf extracts on field performance of cowpea. It was evaluated by using randomized block design (RBD) with nine treatments including control (5% leaf extracts of Calotropis, Papaya, Pongamia, Castor, Moringa, Vitex, Neem, Tulsi and Control) and 3 replications. Seeds were soaked in 5% leaf extracts for four hours and then shade dried to their original moisture content. Crop raised as per POP recommendation of KAU (2016). Observations were recorded on growth and yield parameters during crop growth stages. The results revealed that the performance of seeds treated with organic leaf extracts was better compared to the control. A significant effect was observed in seeds treated with Pongamia leaf extract had given the highest values for plant height (145.07 cm), number of branches per plant (10.27), number of clusters per plant (7.53), number of pods per plant (12.13), seed yield per plot (353.62 g), dry matter production at harvest (56.95 g), and nodule count per plant (17.78). Seeds treated with Calotropis leaf extract had given the highest values for, pod length (17.18 cm) and pod weight (10.63g). Similarly, neem leaf extract had given the highest values for pod girth (2.2 cm) and 100 seed weight (12.88 g). (Experiment-2) The effect of seed treatment with organic leaf extracts on the seedling quality parameters of cowpea was evaluated in a completely randomized design with three replications. Seed treatment was done as in the previous experiment and then shade-dried the seeds for two days, bringing the moisture content to less than 8 %, and packed in a 700-gauge polythene bag kept for storage in ambient conditions. Observations were recorded on seedling quality parameters at the start of the storage, 45 DAS and 3 months after storage. It was observed that seeds treated with organic leaf extracts had better performance compared to control. A significant effect was observed in seeds treated with Pongamia leaf extract had given the highest values for emergence per cent (61.67 %), root length (10.33 cm), seedling dry weight (0.048 g), low electrical conductivity of seed leachate (0.467 dSm -1), emergence index (21.38) and seedling vigour index-II (3.03). Seeds treated with Calotropis leaf extract had given the highest values for germination per cent (63.67 %), low pulse beetle infestation (16.33 %), germination index (324), mean germination time (3.87 days), time taken for 50 % germination (3.34 days) and seedling vigour index-I (2259) by the end of the storage period. Seeds treated with Papaya leaf extract had given the highest values for shoot length (25.65 cm). Similarly, low seed microflora per cent (19.05%), was recorded in seeds treated with neem leaf extract by the end of the storage period. (Experiment-3) The effect of seed treatment with organic leaf extracts on the nutritional attributes of cowpea. Experiments 3 was carried out simultaneously with Experiment 2 by using the same seeds kept for storage in the Experiment 2. Observations were recorded on nutritional attributes which include crude protein, crude fat, crude fibre, vitamin C, calcium content, iron content, ash content, carbohydrate content and phytic acid content at the start of storage, 45 DAS and 90 DAS . A significant effect was observed in seeds treated with Pongamia leaf extract, which had given the highest values for calcium content (48.03 mg/100 g), iron content (2.42 mg/100 g), ash content (4.07 %) and carbohydrate content (51.03 %) by the end of the storage period. Among all the treatments, seeds treated with Pongamia leaf extracts had given the better performance compared to control in field performance as well as improvement in seedling quality parameters and preserving the nutritional attributes of cowpea during the storage period.Item Studies on the C2 generation of the colchicine induced polyploids of chillies (Capsicum frutescens L.)(Division of Agricultural Botany, Agricultural College & Research Institute, Vellayani, 1968) Lathika, M; Aravindakshan, MThe present study was undertaken in the Division of Agricultural Botany, Agricultural College and Research Institute, Vellayani with a view to study the morphological variations that existed in the C2 generation of the colchicine induced tetraploid chillies. Detailed morphological studies of the plants in C2 generation and cytological analysis of the suspected polyploids were conducted. The seeds from the colchicine induced polyploid chillies were carried over to the C2 generation . From the cytological observations made on selected individuals in the C2 population, it was revealed that there occurred 24 tetaploids and three diploids, leading to the conclusion that the C1 tetraploid was possibly a mixoploid. Some of the individuals showed stunted growth in early stages. But most were found to be gigas in size. A general increase in the vigour of morphological characters such as height of plants, spread of plants, number of branches and yield of fruits were found in all cases of polyploids.Item Studies on the effect of growth regulators on germination, growth and oil content in Sesame (Sesamum indicum-L)(Division of Agricultural Botany, Agricultural College & Research Institute, Vellayani, 1963) Santha Kumari, S; Kumara Pillai, PAn experiment was conducted at the Agricultural College and Research Institute, Vellayani during 1962-63, to study the effect of three growth regulators NAA, GA and 2, 4-D on germination, growth and oil content of Sesamum indicum. NAA, GA and 2, 4-D at three different levels 0, 15, 30; 0, 25, 50; and 0, 2, 5 ppm. respectively, were tried for the present study . The three different stages of application were seed, seedling and at the time of flowering . The experiment was conducted as pot-culture in randomised block design with 5 replications and 27 treatment in factorial combinations. Observations on germination percentage, height of plants, number of branches and nodes, fresh weight of stem, number of flowers and fruits, percentage of fruit setting, yield and oil contents of seeds were recorded and the data statistically analysed.Item Studies on the induced polyploids of cluster beans (Cyamopsis P soralioides D. C.)(Division of Agricultural Botany, Agricultural Botany, Agricultural College & Research Institute, Vellayani, 1964) Thankamma Pillai, P K; Kumara Pillai, P