1. KAUTIR (Kerala Agricultural University Theses Information and Retrieval)

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    Metoestrual bleeding and its effect on fertility in natural and induced oestrus in cattle
    (Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 2003) Leeba Chacko; Aravinda Ghosh, K N
    With the objective of studying the occurrence, onset, duration and intensity of metoestrual bleeding and its effect on fertility in natural and induced oestrus in cattle, detailed data of animals belonging to University Livestock Farm and those presented at Artificial Insemination Centre and Bull Station, attached to the Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, were collected using a suitable proforma. Animals with the history of metoestrual bleeding were randomly selected and allotted to the following groups. Group I consisted of 16 animals, comprising- of eight cows and eight heifers, with the history of metoestrual bleeding that were inseminated at natural oestrus. Sixteen animals in group II (eight cows and eight heifers) with the history of metoestrual bleeding were subjected to induction of oestrus on tenth day using 25 mg PGF2 alpha (Lutalyse), which were inseminated at 72 and 96 h after the administration of Lutalyse. Sixteen animals (eight cows and eight heifers) which did not show metoestrual bleeding formed the group Ill. Out of 1626 animals screened, 99 (6.09 per cent) animals exhibited signs of metoestrual bleeding in one or more oestrous cycles. The occurrence was found to be higher in heifers (9.87 per cent) than in cows (4.52 per cent). Higher percentage of Brown Swiss cross bred cattle (9.88 per cent) exhibited signs of metoestrual bleeding, followed by Holstein-Friesian cross bred (7.66 per cent) and Jersey cross breds (7.4 7 per cent). Out of 99 animals with metoestrual bleeding, eight (8.08 per cent) animals showed bleeding on the day of oestrus (day 0), 44 (44.44 per cent) on day 1 of oestrus, 26 (26.26 per cent) on day 2 of oestrus and 21 (21.21) on day 3 of oestrus. Duration of metoestrual bleeding ranged from 6-36 h with a mean of 13.38 ± 2.64 h. Thirteen (13.13 per cent), 57 (57.58 per cent) and 29 (29.30 per cent) animals exhibited scanty, moderate and profuse bleeding, respectively. Thick mucus blood tinged discharge was observed in 60 per cent of cases, which on microscopic examination revealed fern pattern, that lay scattered in disintegrated form with presence of RBCs, in stained smears. Thin mucus blood tinged discharge was noticed in 40 per cent of cases with presence of RBCs in stained smears, without fern pattern. Microbial growth was not detected when metoestrual discharge was inoculated on the culture media and incubated at 37°C for 24 h. Gram positive bacilli were detected as the contaminants after 48 h. Out of eight cows and eight heifers in group II subjected to PGF2alpha administration, seven (87.5 per cent) and eight (100 per cent) responded to treatment, respectively. The overall oestrus response was 93.75 per cent. The time taken for the induction of oestrus in cows was 66.14 ± 1.55 h as against 59.88 ± 1.66 h in group II heifers. Average duration of oestrus in cows and heifers was 25.13 ± 0.99 and 18.75 ± 0.53; 31.43 ± 1.13 and 28.75 ± 2.00, and 23.63 ± 2.27 and 19.38 ± 0.65 h for groups I, 11 and III, respectively. Analysis of data revealed significant difference in the duration of oestrus in cows and heifers in group II from that of cows and heifers in groups I and III (P37.5 per cent of cows and 12.5,37.5 and 50 per cent heifers showed high, medium and low intensity of oestrus, respectively. In group II the corresponding values were 57.14, 28.57 and 14.28 per cent in cows and 62.5, 25 and 12.5 per cent in heifers. In group III 12.5, 37.5 and 50 per cent cows and 25, 37.5 and 37.5 per cent heifers exhibited high, medium and low intensity of oestrus, respectively. Out . of eight cows and eight heifers induced, none exhibited metoestrual bleeding in the induced heat. The overall conception rate in groups I, Il and III were 18.75, 66.66 and 43.75 per cent, respectively. It can be recommended that induction of oestrus using prostaglandin can be employed for enhancing the conception rate in animals with the history of met oestrual bleeding.
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    Classification and characterization of follicular oocytes of crossbred cattle
    (Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 2001) Lydia Priscilla, K; Balakrishnan, P P
    The objective of the present study was to analyse the ovarian factors that would influence the type of oocyte recovered for further use in terms of in vitro embryo production. The normal ovaries of crossbred cows collected randomly from the slaughter house were classified into four stages Stage I (SI), Stage Il (S2), Stage III (S3) and Stage IV (S4) of the oestrous cycle according to the visual appearance of CL and ovarian morphology. The length, width, thickness and weight of the ovary were significantly influenced by the stage of the oestrous cycle and the maximum value was recorded during the S3 stage (late luteal phase) of the cycle. The average values recorded irrespective of the stage for length, width, thickness and weight of the ovary was 2.47±O.03cm, 1.81±O.03cm, 1.42±O.03cm and 4.05±O.14g respectively. The mean number of vesicular follicles belonging to the 4 to 8mm category was significantly influenced by the stage of the oestrous cycle (p8mm diameter follicles were not influenced by the stage of the oestrous cycle. The late luteal phase (S3) had more number of follicles in less than 4mm category whereas S3 and S4 stage had more number of follicles in 4 to 8mm and >8mm category. The size of the follicle had an interactive effect along with the quality of oocyte obtained in each category of the follicle. The number of oocytes obtained from <4mm sized follicle was significantly high (pcategories. The good quality oocytes were also found to be significantly higher in number when recovered from <4mm sized follicles. The recovery rate of oocytes as per the stage was found to have a statistical significance (phigher output from ovaries belonging to the S3 phase than the. number obtained from SI, S2 and S4 stage. Moreover, the quality of the oocyte recovered from S3 stage was significantly influenced (pstatistically similar in their effect on oocyte quality. Slicing method yielded the maximum number of oocytes per ovary than when compared to the other techniques employed. There was significant difference in the recovery rate among the methods (pboth in number and quality (6.27 and 61.53 per cent of grade I). The mean number of oocytes recovered by aspiration, puncturing and post aspiration slicing was 4.16, 3.67 and 3.13 respectively. The method of recovery and the quality of oocyte recovered had an interactive and significant effect on the oocyte recovery rate (pThe level of sodium, potassium, iron and copper in the follicles of various diameters present during the different stages of the cycle in the ovaries was found to vary significantly between stages. The level of zinc was not influenced by the stages of the cycle. The variation in the concentration of the minerals was found to be related with the normal physiological changes that occurred during the different stages of the cycle.