2. Institutional Publications

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Author: search.filters.author.Sreekandan Nair, G

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    Screening of black pepper (Piper nigrum L.) calliclones for phytophthora foot rot resistance / tolerance
    (Kerala Agricultural University, 1996) Shylaja, M R; Sreekandan Nair, G; James Mathew
    Calliclones of different black pepper cultivars viz., Kalluvally, Cheriakanyakkadan, Balankotta, Karimunda and Panniyur-1 were regenerated from calli, screened against toxic metabolite(s) of Phytophthora capsici. The regenerants derived from screened and unscreened calli were further tested for resistance / tolerance to P. capsici using different methods of screening viz., natural screening (keeping in infected field), screening by electrolyte leakage method and screening by artificial inoculation with culture disc of P. capsici. None of the regenerated calliclones was found to be completely resistant to the disease in natural screening. However, some of the regenerants derived from unscreened calli exhibited higher degree of tolerance to the disease revealing the possibility of exploiting somaclonal variation for Phytophthora foot rot disease screening in black pepper. The calliclones of Cheriakanyakkadan recorded greater degree of tolerance to the disease as compared to others.
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    Isoenzyme variation and species relationship in genus piper
    (Kerala Agricultural University, 1996) Abraham Sebastian; Sujatha, V S; Nybe, E V; Sreekandan Nair, G; Augustine, A
    Eleven species of Piper including Piper rugrum were studied for variations in isoenzymes of three enzymes viz., peroxidase, esterase and glutaraate oxaloacetate transaminase. On grouping based on isoenzyme similarity, P. nigrum Linn.. P. pseudonigrum Velayudhan and Amalraj, P. bababudani Rahirnan and P. galeatum DC formed one group while P. argyrophyllum Miq. and P. attenuatum Buch-Hara constituted the second group and P. chaha Hunter, P. hapnium Miq. and P. colubrinum Link emerged as the third group. P. betle Linn, and P. longum Linn, showed their distinctness from the rest of the species. Least similarity was observed between P. colubrinum on one side and P. pseudonigrum and P. bahabudam on the other side.
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    Seed set in ginger (Zingiber officinale rosc.) through in vitro pollination
    (Kerala Agricultural University, 1996) Valsala, P A; Sreekandan Nair, G; Nazeem, P A
    Different in vitro pollination techniques viz., stigmatic, srylar, intra-ovarian, placenta!, modified placental pollination and test-tube fertilization were tried in ginger flowers. The pollen grains suspended in ME, medium were used for pollination. The pollinated pistil or its part was cultured in the medium of 1/2 MS + NAA 0.5 mg 1' + BAP 2.5 mg 1 ' + coconut water 15 per cent v/v. Seed development was obtained in placental, modified placental pollination and test tube fertilization. The seed germinated under in vitro condition on supply of appropriate combination of 2,4-D, BAP and NAA
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    In situ green manure production as mulch material for ginger
    (Kerala Agricultural University, 1997) Alice Kurian; Sreekandan Nair, G; Valsala, P A
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    Effect of toxic metabolite(s) of phytophthora capsici on various stages of morphogenesis of black pepper calli
    (Kerala Agricultural University, 1997) Shylaja, M R; Sreekandan Nair, G
    The effect of toxic metaholite(s) of Phytophthora capsici on various stages of morphogenesis of black pepper calli was studied. The survival rate of the call! in toxin medium was influenced by the varieties / cultivars used for the study. Once the calli survived in the toxin medium, the toxic tnetabolite(s) did not inhibit further growth of the calli. The shoot proliferation and elongation were also not influenced by the metabolite(s) in the media. However, the root growth was affected adversely.
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    In vitro production of toxic metabolite(s) by phytophthora capsic1 and partial purification of the metabolite(s)
    (Kerala Agricultural University, 1997) Shylaja, M R; Sreekandan Nair, G; Augustine, A; James Mathew
    Phytophthora capsici, the causal organism of Phytophthora foot rot disease in black pepper produces toxic metaholite(s) under in vitro conditions. Maximum accumulation of toxic metabolite(s) was observed in shake cultures of 15 days incubation in Ribeiro's medium. The symptoms induced by toxic metabolite(s) were quite typical to symptoms of natural and artificial infection by the pathogen. The toxic metabolite(s) accumulated in the in vitro culture was found to be heat stable aim non-specific. The toxic metabolite(s) could not be separated using organic solvent fractionation since it is present in the aqueous fraction of the culture filtrate. However, ion exchangers like Dowex 1 and Dowex 50 could be used for separating the metabolite(s) from the aqueous fraction.
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    Isoenzyme variation in Piper nigrum L.
    (Kerala Agricultural University, 2000) Abraham Sebastian; Sujatha, V S; Nybe, E V; Sreekandan Nair, G; Mallika, V K
    Isoenzyme patterns of 26 varieties of P. nigrum were compared for three enzymes viz., csterase, peroxidase and glutamate oxaloacetate transaminase. Similarity among the varieties ranged from 0.40 1.00. Wide variation was observed among the cultivated types of P. iiignini for the three enzymes analysed.
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    Quantification of medicinally valuable weeds in oil palm plantations of Kerala
    (Kerala Agricultural University, Vellanikara, 2002) Sarada, S; Sreekandan Nair, G; Reghunath, B R
    The medicinally valuable weeds naturally growing in young, medium and mature plantations of oil palm were identified and quantified by stratified random sampling technique. Quantification of plants was done in comparison with that in the open condition. Chrysopogon aciculatus Trin. dominates in all the four strata. Naregamia alata Wight & Arn. is another dominant species in mature plantation. Hemidesmus indicus R. Br. occurs frequently in all the four strata. Another frequently occurring species in young oil palm plantation is Elephantopus scaber Linn, and in medium plantations is Chromolaena odorata King & Robinson. Phyllanthus amarus Schum.& Thonn. is very frequent in medium plantation and open condition. Holostemma adakodien Schult occurs frequently in mature plantation. Young oil palm plantation and open condition were found to be the most similar strata whereas mature plantation and open condition were the most dissimilar strata in vegetation pair-wise analysis. Medium oil palm plantation was found to have high concentration of dominance. Species diversity was the highest in mature oil palm plantation. Species evenness was maximum in open condition.