2. Institutional Publications

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Subject: search.filters.subject.micropropagation

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    Temporary immersion bioreactor for commercial micropropagation
    (Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Kerala Agricultural University, 2020) Waghmare, Vaibhav Gautam; Shylaja, M R
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    In vitro shoot regeneration from axillary bud cultures of malabar white pine (Vateria indica L.) through tissue culture
    (Kerala Agricultural University, 1997) Ashok B Devatar; Vijayakumar, N K
    Axillary buds collected from seedlings of Veteria Mica L., the recalcitrant Malabar white pine, were cultured under in vitro conditions with the objective of standardising the micropropagation technique for this species. A few media combinations consisting of full and half strength mineral salts of Murashige and Skoog (MS) as well as woody plant medium (WPM) have been identified to be capable of supporting culture establishment and bad break. Among these, the media containing half strength mineral salts of MS supplemented with organic nutrients and growth regulators alone supported elongation and continued growth of shoots. Shoots were 1-2 cm in height with 2-3 leaves in eight weeks under controlled conditions with 16 h photoperiod at a temperature of 27+2" C. The success obtained is the first of this kind in this species.
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    In vitro propagation of Rose cv. Folklore
    (Kerala Agricultural University, 1998) Wilson; Nayar, N K
    Surface sterilisation, stage of explant, media for culture establishment and multiple shoot induction were standardised for in vitro propagation of rose cv. Folklore. Treatment with mercuric chloride 0.08% for 12 min was found to be optimum for the surface sterilisation of axillary bud explants. Axillary buds excised four days after flower opening, having 1.0 cm length, exhibited the best response in culture establishment. The Murashige and Skoog (MS) basal medium supplemented with benzyl aminopurine (BAP) 2.5 mg I 1 + 2.4 dichlorophenoxy acetic acid (2,4-D) 0.5 mg 1' was found to be the suitable medium for culture establishment. The best combination for multiple shoot induction was MS basal medium supplemented with kinetin 2.0 mg 1' + gibberellic acid (GA,) 1.0 mg 1'.