Enzyme characterization of the acid sulphate soils of Kuttanad

Abstract

Regarding the enzyme status of the acid sulphate soils, the activity of urease was the highest in Thakazhi (L4-75.78 ppm of urea hydrolyzed g-1 soil h-1) in the surface layer. Acid and alkaline phosphatase activities were found to be the highest in Purakkad (L3-57.58 μg pnp released g-1 soil h-1) and Thakazhi series (L4-46.41 μg pnp released g-1 soil h-1) respectively in the surface layers. The highest activity of dehydrogenase was noticed in Thuravur series (L6-115.74 μg of TPF released g-1 soil 24 h-1) in the surface and Kallara series in the subsurface layers (L2-55.39 μg of TPF released g-1 soil 24 h-1). Karappadam and Kayal recorded the values of 145.73 and 114.80 μg of TPF released g-1 soil 24 h-1 for dehydrogenase activity respectively at surface layers. With regard to the microbial population, Thottappalli (L5) recorded the highest total bacterial count and fungal population in both surface and subsurface layers. Thiobacillus spp. recorded the highest population of 9.08 log cfu g-1 of soil at Kallara series (L2) while P solubilizers recorded the highest count at Ambalapuzha series (L1). Thuravur series recorded the highest actinomycetes population in subsurface soils. Micahelis – Menten constant, Km and the maximum velocity Vmax for different enzymes were determined and it was observed that for dehydrogenase Vmax value was found to be the highest in 6th week and for phosphatase only after 12th week of incubation. Thematic maps were prepared based on themes like microflora and enzyme status using GIS (ARC VIEW). Hence the study conclude that, with respect to biological fertility status of the study area, Purakkad recorded the highest enzyme activity number (23.69) at 0-15 cm, while Kallara series recorded the highest enzyme activity number in the subsurface layer and are observed to be biologically sustainable.