Differentiation of beef from chevon by serological methods

Abstract

Identification of meat of different species by physical examination alone is difficult. Serological tests are advocated as one of the reliable methods. In order to differentiate beef from chevon, tube precipitation and gel-diffusion tests were concurrently done. Antisera raised in rabbits against beef and chevon, and saline extracts of meat samples collected from known sources as antigen, were used for the tests. In order to remove cross-reacting antibodies, the sera were absorbed with freese – dried antigen against which cross- reacting antibodies were present.

Tube precipitation and gel-diffusion tests were adopted for identifying the meat samples. The tests conducted on eighty samples of meat gave cent percent accuracy in identifying the meat.

Buffalo meat and mutton used in the test as antigen reacted in the same manner as that by beef and chevon respectively. It was also possible to detect the presence of adulterant in a mixed sample of beef and chevon when the proportion or level of adulterant was up to 20 percent.

The antisera could be preserved for more than six months at 50C and-200C without loss of efficacy inspite of occasional electricity failure. It is concluded that tube precipitation and gel-diffusion tests are reliable methods for differentiation of beef and chevon.