1. KAUTIR (Kerala Agricultural University Theses Information and Retrieval)
Permanent URI for this communityhttp://localhost:4000/handle/123456789/1
Browse
5 results
Search Results
Item Antioxidant and anticarcinogenic potential of jackfruit based ready-to-cook (RTC) curry mixes(Department of Community Science, College of Agriculture,Vellayani, 2019) Gayathri Mohan; Suma DivakarThe research work entitled, “Antioxidant and anti-carcinogenic potential of jackfruit based Ready-To-Cook (RTC) curry mixes” was conducted at College of Agriculture, Vellayani during 2017-2019, with the objective to ascertain the antioxidant and anti-carcinogenic properties of the jackfruit based curry mixes (‘Avial’ mix, ‘Koottu’ mix and ‘Ularth’ mix) which were developed earlier at the Department of Community Science, using raw jackfruit (Koozha type). Methanol, ethanol, petroleum ether and acetone were used to extract the bioactive compounds of the powdered mixes for phytochemical screening, antioxidant analysis and verification of anti-carcinogenic property. The results of the phytochemical analysis indicated the presence of tannins, flavonoids, saponins, steroids, phenolic compounds, cardiac glycosides and anthraquinones in the three mixes; whereas the analyses revealed the absence of alkaloids and phlotobatinins. Quantitative analysis of antioxidants revealed that the beta carotene content of the RTC mixes was in the range of 0.07-1.16 mg/100g. Beta carotene content was found to be more in ‘Avial’ mix (1.16mg/100g). Ascorbic acid content was also found to be higher for ‘Avial’ mix (38.45 mg) and the lowest content was found for ‘Ularth’ mix (15.34 mg). The saponin content was found to be highest in ‘Avial’ mix (6.65 per cent) and lowest in ‘Ularth’ mix (5.00 per cent). The tannin content of ‘Ularth’ mix (11.57 mg) was observed to be on par with ‘Avial’ mix (11.52 mg). Total phenol content was found to be more in ‘Koottu’ mix (21.53 mg/100g) and the total flavonoid content of RTC mixes was seen to be in the range between 0.86-3.25 mg/100g. There was significant difference in the lectin content of ‘Avial’ mix (0.35%), ‘Koottu’ mix (0.56%) and ‘Ularth’ mix (0.75%). Alkaloids and selenium were found to be absent in the RTC mixes. In case of mineral analyses, Copper content was found to be higher in ‘Ularth’ mix (2.57 µg); zinc content was in the range of 4.65-6.55 µg and was found to be higher for ‘Koottu’ mix (6.55 µg) and lower for ‘Ularth’ mix (4.65 µg). Manganese content was found to be higher in ‘Koottu’ mix (5.30 µg) while, it was observed to be on par in ‘Avial’ mix (2.55 µg) and ‘Ularth’ mix (2.51 µg). The highest iron content was found in ‘Ularth’ mix (0.92 µg). Antioxidant activity in the present study revealed that ‘Avial’ mix had the highest DPPH activity with an IC50 value of 33.81 µg/ml. Total antioxidant activity was found to be more for ‘Avial’ mix (41.44 per µg/ml), followed by ‘Koottu’ mix (42.41 µg/ml) and ‘Ularth’ mix (43.45 µg/ml). ‘Avial’ mix showed more ferric reducing capacity while ‘Ularth’ mix had the least capacity in this regard. ABTS radical scavenging activities of RTC mixes ranged between 34.84-46.69 µg/ml. ABTS radical scavenging activity was observed to be higher for ‘Avial’ mix (34.84 µg/ml) and lower values were noted for ‘Ularth’ mix (40.52 µg/ml). Hydroxyl radical scavenging activity of RTC mixes was found to range between 50.55-52.55 µg/ml. ‘Avial’ mix showed higher superoxide radical scavenging activity with an IC50 value of 48.54 µg/ml and the lowest superoxide radical scavenging activity was observed in ‘Ularth’ mix (60.73 µg/ml). However, the highest nitric oxide scavenging activity was observed for ‘Ularth’ mix (14.11 µg/ml) followed by ‘Koottu’ mix (30.92 µg/ml) and ‘Avial’ mix (36.58 µg/ml). Among the three jackfruit based Ready-To-Cook (RTC) curry mixes ‘Avial’ mix was found to have higher antioxidant property and was therefore taken up for studying the anti-cancer property. Cytotoxicity of ‘Avial’ mix was estimated by MTT assay on Human breast adenocarcinoma cells (MCF-7) and 17 per cent of loss in cell viability was observed. Findings of the present study revealed that the jackfruit based RTC mixes comprised of various health promoting components such as beta carotene, ascorbic acid, saponins, tannins, total phenols, total flavonoids and lectins they also had potent antioxidant activities. Thus the curry mixes, in particular the ‘Avial’ mix can be promoted as functional food which will help to enhance its commercialization.Item Development of osmo dehydrated red banana(musa spp)(Department of post harvest technology, college of agriculture, Vellayani, 2019) Archana, A K; Geetha Lekshmi, P RItem Oxidative stress and protein profiling in sweet potato (Ipomoeabatatas (L.) Lam.) under abiotic stresses(Department of Plant Biotechnology, College of Agriculture, Vellayani, 2017) Aiswarya, N S; Ravi, VThe present study an “Oxidative stress and protein profiling in sweet potato (Ipomoeabatatas (L.) Lam.) under abiotic stresses” was conducted during the period 2014-2015 in the Division of Crop Production, Central Tuber Crops Research Institute (CTCRI) Thiruvananthapuram. The objective of the study was to elicit information on the antioxidative enzyme activity, protein profiling, growth parameter and yield parameter of five varieties / genotypes of sweet potato under irrigated high temperature stress (HTS) (40oC during the day time) and water deficit stress (WDS) ( 2nd and 3rd month after planting) conditions and identify marker physiological parameter which can be used for identifying / developing sweet potato varieties for tolerance to abiotic stress conditions. The study indicated that the vein length of five different sweet potato varieties/ genotypes was not significantly affected by HTS and WDS. The leaf production of five sweet potato varieties was significantly affected by both HTS and WDS. The leaf retention of five varieties / genotype was differentially affected by both heat and water stress. There was negligible difference in yield parameters between stress and control condition. However, there was significant difference in yield observed for parameters among five varieties. The effect of abiotic stress on various physiological parameters like Relative water content (RWC), chlorophyll and carotenoid content significantly differed in control plant. Among the WDS and HTS treatments, the chlorophyll and carotenoid content decreased in all the five varieties. Chlorophyll and carotenoid content were significantly reduced in plants exposed to HTS. RWC of leaves varied under both WDS and HTS conditions. A higher RWC was observed in plants exposed to HTS condition compared to plants was WDS condition. Total soluble protein content of leaf was found to be lower due to presence of high mucilage in leaves. Total soluble protein in tuber is increased in control and WDS conditions. Total soluble protein in tuber forming roots had increased under stress free condition. The effect of abiotic stress on biological parameter like proline status of plants under stress conditions followed an increasing trend compared to stress free condition. The outcome of abiotic stress on various enzymatic antioxidant such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), ascorbate reductase, glutathione reductase (GR) was studied. Among the five different sweet potato varieties/ genotypes (Sree Arun, S-1464, S-1466, Sree Kanaka and Kanjangad) S-1466 and Sree Kanaka had maximum GR activity under HTS compared to WDS and control condition. Catalase activity of five varieties/ genotypes of sweet potato plant varied among different stress condition (WDS and HTS) compared to control. The GR activity was significantly reduced due to stress condition and SOD activity increased under stress conditions. The APX enzyme activity is more under WDS conditions compared to HTS and control conditions. Ascorbate reductase become there was no change in absorbance therefore the enzyme activity could not be measured. The AA content in sweet potato leaves increased under WDS condition compared to HTS and control. The glutathione content in sweet potato leaves was negligible or below detectable amount under both control and stress condition. Under HTS condition SOD activity increased in Sree Arun and S-1466 alone whereas other varieties/genotypes had increased SOD activity under WDS. Kanjangad had maximum APX activity in HTS whereas S-1464 had higher APX activity in WDS. From the present study, it is inferred that SOD activity can be utilised as biochemical marker for developing HTS tolerant varieties as it showed consistent changes under both WDS and HTS. APX activity can be utilised as marker for identifying WDS tolerant varieties. Detailed molecular biochemical and physiological information will be necessary using different varieties for getting appropriate activities as the marker to screen abiotic stress tolerant varieties.Item Physiological and molecular analyses of growth responses in black pepper (piper nigrum L.) under elevated carbon dioxide environments(Department of plant physiology, College of agriculture, Vellayani, 2015) Minu, M; Manju, R VItem Development of nendran banana chips with enhanced shelf life and quality(Department of Processing Technology, College of Agriculture, Vellayani, 2014) Sonia, N S; Mini, C (Guide)