PhD Thesis

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    Fungal diseases of selected medicinal plants of Kerala
    (Department of Plant Pathology, College of Horticulture, Vellanikkara, 1991) Sukumara Varma, A; Abi Cheeran
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    Technological evaluation of kera ice cream
    (Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 1996) Geevarghese, P I; Francis, U T
    An experiment was conducted to assess the suitability of incorporating coconut fat in the form of coconut cream in preparing ice cream and ice cream mix powder replacing milk fat at 25, 50, 75 and 100 per cent levels (treatments) so as to reduce the cost of preparation of ice cream. The product developed was named as kera ice cream and the qualities were compared with normal ice cream (control). Kera ice cream was prepared with pineapple flavour and a combination of sodium alginate and glyceryl monosterate as stabilizer since it was found to be the most suitable. It was found that replacement of milk fat with coconut fat at any level does not influence the acidity, pH and specific gravity of kera ice cream mix. The relative viscosity of kera ice cream was increased as fat substitution level increased. Higher surface tension was observed for the control, and among treatments it showed a gradual increase as replacement level increased. It was observed that the overrun percentage increased as replacement with coconut fat increased. Contrary to this a decreasing trend in meltdown time was observed as percentage replacement with coconut fat increased. Whipping ability was lowest for the control during the first 5 min of freezing but it significantly increased during the second five min of freezing. Structural details of the ice cream revealed that as replacement level increased the air cell diameter and cell wall thickness increased. Organoleptic quality of kera ice cream were comparable to normal ice cream. The savings in cost of production for kera ice cream with 100 per cent replacement was calculated as 40.57 per cent compared to control. The properties of the reconstituted ice cream revealed more or less similar trend in characters like acidity, pH, specific gravity, overrun, meltdown time and organoleptic properties as that of the freshly prepared ice cream. The ultra structure of the kera ice cream mix powder particle were studied and it revealed that as replacement level increased the particle size also increased. Clumping of the particles and irregular surface were more evident at 75 and 100 per cent level whereas particles of the control and 25 per cent had smooth surface. Solubility index of the powder increased as percentage replacement increased. Significant difference could be noted with regard to bulk density and percent volume occupied by the powder particle. Storage studies were conducted with different packaging material and metallised polyester polyethylene was found to be the best. The moisture and titratable acidity of kera ice cream mix powder with and without antioxidant were significantly higher at any replacement level at 180 days of storage. The thiobarbituric acid value showed a significant difference for the powder without antioxidant at 120 days of storage and with antioxidant the difference could be noted at 180 days of storage. Peroxide value was recorded as zero at different periods of storage upto 180 days of storage. At 180 days of storage, powder without BHA at any replacement level showed significant difference whereas in antioxidant added powder difference could be noted at 75 and 100 per cent replacement levels. Nutritional qualities of kera ice cream was evaluated by rat feeding trials and was found that protein efficiency value and feed efficiency were comparable to ice cream diet. The cholesterol and triglyceride level in animals fed with kera ice cream and ice cream were also not different. Pathological examination of carcases and tissues of animals under treatment and control groups did not reveal significant changes indicating that incorporation of coconut fat cannot cause any untoward effect in organs and tissues of animals. It can be concluded that coconut fat can be incorporated in the prepartation of ice cream and mix powder with out any noticeable changes in the quality. Addition of antioxidant prolongs the keeping quality of the powder by two months. There is no health hazard in consuming the kera ice cream as revealed by the pathological examination of tissues from the experimental animals fed with kera ice cream.
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    Qualitative changes of yoghurt prepared from milk preserved by different methods
    (Department of Dairy Science, College of Veterinary and Animal Sciences,Mannuthy, 1990) Prasad, V; Sukumaran, M V
    An experiment was conducted to study the qualitative changes of yoghurt prepared from milk preserved by different methods. An attempt was also made to study the efficiency of LP system in controlling psychrotrops in refrigerated milk thereby extending the keeping quality of such stored milk. The efficacy of the system was compared with pasteurized milk and raw milk stored under similar conditions. An exhaustive review of literature has been presented on the use of various preservation methods employed in milk, quality of yoghurt and other related aspects. The methods of analyses of some important components of milk and yoghurt has been detailed. The milk after collection were divided into three portions and used for various treatments A (raw milk as such), B (laboratory pasteurized) and C )LP activated). Samples in each treatment were again divided into four parts and stored under refrigeration for 0, 24, 48 and 72 hours for further analysis and yoghurt preparation. When raw milk was stored under refrigeration, acidity developed to a significantly high level (P < 0.05) at the end of 72 hours of storage whereas no significant increase in activity was noticed in treatments B and C. This result was corroborated by a decrease in both psychrotrophic and total bacterial counts in milk under treatments B and C. So it was concluded that the development of acidity in treatment A could be due to bultiplication of microbes particularly psychrotrophs. In B and C its growth was arrested by the treatments. Hydrogen peroxide and thiocyanate were detected in all raw milk samples at a level of 3.78 and 7.33 ppm respectively. The level of H2 O2 and SCN – used for the activation of LP system for the study were found to be adequate, since the psychrotrophic count in milk at the end of 72 hours in treatment C was less than that found in treatment B. The residual H2 O2 and SCN – contents at the end of 72 hours were found to be within the normal level found in milk thus having no health hazards, by this method of preservation. When yoghurt samples were prepared from stored milk no significant differences between treatments were noticed in the values of fermentation period, acidity, pH, total proteins and total solids. A significant increase in NPN and tyrosine values were noticed in yoghurt prepared from milk under treatment A indicating extensive proteolysis in milk during storage under this treatment. However, no difference was noticed between treatments B and C. Diacetyl and acetaldehyde were produced at a desirable level in yoghurt samples under all the three treatments. A proper ratio of Str. Thermophiles – 6 and L. bulgaricus – 4 was found to be maintained in all yoghurt samples. On organoleptic evaluation the yoghurt prepared from milk under treatment C could not be distinguished from other two treatments. In fact, yoghurt under C got a higher total score on organoleptic evaluation than A and B. Low score for body and texture, and falvour was observed under treatment A. This may be attributed to the effect of growth of psychrotrophs in raw milk during refrigeration. From the results, it was concluded that good quality yoghurt can be prepared from milk stored under refrigeration following pasteurization or LP activation. Yoghurt samples prepared from milk stored under treatment A was found to be of inferior quality when compared to B and C. The result also confirmed that LP system can be recommended as a safe preservative for extending storage life of refrigerated milk. When such stored milk was used for yoghurt production, no significant difference in the quality was noticed when compared to yoghurt under treatment B. Suggesting that LP activated milk can be conveniently and economically used for the production of fermented milk products like yoghurt without any apparent harmful effect.
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    Survival of Xanthomonas campestris pv. oryzae and its control in Kuttanad
    (Department of Plant Pathology, College of Agriculture, Vellayani, 1996) Mary, C A; Sasikumar Nair
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    Structure and function of the shell gland in Japanese quail under different levels of dietary calcium
    (Department of Physiology and Biochemistry, College of Veterinary and Animal Sciences,Mannuthy, 1994) Philomina, P T; Ramakrishna Pillai, M G
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    Land evaluation and suitability rating of the major soils of Onattukara region
    (Department of soil science and agricultural chemistry, College of Agriculture, Vellayani, 1998) Premachandran, P N; Subramonia Iyer, M
    The need for a scientific approach in inventorying and utilizing land resources most economically and efficiently is now universally accepted. Agricultural prosperity, to a great extent, depends on judicious use of soils and rational application of soils data. A reconnaissance soil survey of Onattukara region was undertaken to study the extent and distribution of the different soils for soil classification, land capability and irrigability classification and for subsequent land evaluation. The study reveals that Onattukara region extends over an area of 40,948 ha. The soil map showing the distribution of the identified twenty soil series has been prepared. The climatological data reveals that the soil moisture regime is , ustic and the soil temperature regime is isohyperthermic. The soils have been classified as per Soil Taxonomy (U.S. Soil Survey Staff, 1975) and Keys to Soil Taxonomy( U.S. Soil Survey Staff, 1994 and 1996). Ten series were classified under Entisols, SIX under Inceptisols, three under Ultisols and one under Alfisols. Based on the inherent soil characteristics and landscape features, the soils have been grouped into three land capability classes, nine capability subclasses and land capability map prepared. The soils were classified into five land irrigability classes, eight land irrigability subclasses and land irrigability map prepared. The soils were evaluated based on principles of land evaluation and rating of productivity parameters. The productivity rating of the soil propertIes agamst the productivity index for nee, coconut, sesamum ,cassava and banana were made and conclusions arrived at. Productivity rating shows that Kottakakam series is best suited for rice, Palamel, best for coconut and banana, Artuva and Mahadevikad, for sesamum and Palamel and Vallikunnam, for cassava. On the basis of the studies carried out, a land use is proposed for the twenty soil series of Onattukara region based on crop suitability ratings and crop suitability map prepared for the five crops taken for study. A proper soil survey interpretation provides information on soil potential, productivity and limitations in their sustained use. Soil survey information forms the major basis for land evaluation. A thorough knowledge of the potentialities and limitations of every piece of land is a prerequisite in its efficient utilization. A systematic survey is essential for the evaluation and classification of the soils based on their inherent soil characteristics, land capability, land irrigability and land suitability. A systematic survey and evaluation of the soils of Onattukara region was taken up to study, interpret, classify and to show their location and extent on base maps. It is hoped that the present study would open up avenues for further investigations on land evaluation, crop suitability and other management aspects for sustained use of soil resource data to the best advantage. This will also form the basis for microlevel planning for integrated and sustainable development of the region under Panchayat raj.
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    Management of foot rot of black pepper (piper nigrum L.) with va mycorrhiza and antagonists
    (Department of Plant Pathology, College of Agriculture, Vellayani, 1998) Christin Robert, P; Sivaprasad, P
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    Optimization of shade, nutrients and growth regulators for cut-flower production in anthurium
    (Department of Pomology and Floriculture, College of Horticulture, Vellanikkara, 1997) Salvy, B R; Valsalakumari, P K
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    Leaf litter dynamics in acacia and eucalyptus plantations
    (Department of soil science and agricultural chemistry, College of Agriculture, Vellayani, 1997) Moossa, P P; Venugopal, V K
    An investigation entitled “Leaf litter dynamics in Acacia and Eucalyptus plantations” was undertaken to study the leaf litter production, litter decomposition and nutrient release characteristics, and impact of Eucalyptus and Acacia monoculture plantations on the soil physico-chemical and biological characteristics during 1994-97. One hectare each of Acacia auriculiformis, Eucalyptus tereticornis and a moist deciduous forest coming under the Kulathupuzha range of Kerala Forest Department were selected for the study. Field experiment were conducted to determine the leaf litter production and decomposition characteristics of plantation and natural forest. Leaf litter collected at monthly intervals showed that highest litter production was in Acacia (9.4 t ha-1 year-1) followed by natural forest (6.67 t ha-1 year-1) and Eucalyptus (4.68 t ha-1 yaer-1). Pattern of litter production was unimodal in Acacia and natural forest while in Eucalyptus it was bimodal. Chemical analysis of litter samples of plantations and natural forest revealed that with respect to nutrient content fresh and leaf litter of Acacia and Eucalyptus is superior to natural forest whereas litter quality in terms of water soluble components, polyphenols, lignin and cellulose were superior in natural forest. Annual dry matter loss of leaf litter by decomposition followed the order natural forest > Acacia > Eucalyptus. Nutrient release pattern of major and micro nutrients were also worked out for the leaf litter during the process of decomposition and natural forest litter recorded the highest mobility for all the nutrients on decomposition. Improvement in soil physico-chemical properties were noticed due to leaf litter decomposition in the monoculture plantation and natural forest. Biological activity in terms of earthworm and nematode under monoculture plantation was significantly lower than that of adjacent natural forest. Microbial content of soil below the litter bag during different months of decomposition were low in Eucalyptus and Acacia. Chemical analysis of the profile samples of various plantations revealed lower nutrient status in respect of major and micronutrients compared to natural forest. The physical properties of the soil also recorded as unfavourable change in the monoculture plantations as compared to the adjacent natural forest. Proximate anlysis of soil organic matter and humus characterization of the soil organic matter separated from various plantations indicated a low rate of humification and condensation under Eucalyptus. Low humic acid and fulvic acid content in humus and dominance of fulvic acid over humic acid was also noticed in Eucalyptus plantations. Functional group analysis of humic acid and fulvic acid showed that humic material separated from natural forest contained higher quantity of total acidity, contributed by COOH and phenolic group compared to plantation soils. UV, IR, DTA and TGA analysis of humic acid and fulvic acid separated from various plantations showed no variation with respect to natural forest. Allelopathic effect of fresh leaf and leaf litter extract of various plantations on the germination and growth of rice and cowpea followed the order Eucalyptus > Acacia > natural forest and as the concentration decreased from 1: 2 to 1: 10, the allelopathic inhibition also decreased significantly. Allelopathic effect of soil extract also followed the order Eucalyptus > Acacia > natural forest. Thus it can computed that though the leaf litter production and litter quality of monoculture plantations are comparable with that of natural forest, leaf litter decomposition and nutrient released were significantly lower than that of natural forest. Soil physico-chemical properties, soil fertility and biological activities were also adversely affected by monoculture plantations.
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    Response of cucumber (Cucumis melo L.) to drip irrigation under varying levels of nitrogen and potash
    (Department of Agronomy, College of Agriculture, Vellayani, 1997) Lakshmi, S; Sasidhar, V K