PhD Thesis
Permanent URI for this collectionhttp://localhost:4000/handle/123456789/48
Browse
9 results
Search Results
Item Management of foot rot of black pepper (piper nigrum L.) with va mycorrhiza and antagonists(Department of Plant Pathology, College of Agriculture, Vellayani, 1998) Christin Robert, P; Sivaprasad, PItem Somaclonal variation in black pepper (piper nigrum L)(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 1996) Shylaja, M R; Sreekandan Nair, GInvestigations on the exploitation of somaclonal variation for screening for resistance to Phytophthora foot rot disease in black pepper were carried out at the Plant Tissue Culture Laboratory of the Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara during September 1991 to January 1995. Calliclones of different black pepper cultivars viz. Kalluvally, Cheriakanyakkadan, Balankotta, Karimunda and Panniyur – 1 were produced with and without applying in vitro selection pressure using toxic metabolite(s) of Phytophthora capsici. In vitro induction of mutation using gamma irradiation and partial purification of the toxic metabolite(s) present in the culture filtrate of P. capsici were also attempted in the present study. Leaf puncture bioassay of the concentrated culture filtrate (CCF) of P. capsici showed that toxic metabolite(s) were accumulated in the culture filtrate. The symptoms produced by CCF were quite typical of natural and artificial infection by P. capsici. Concentrated culture filtrate induced quick electrolyte leakage from leaves and calluses. Concentrated culture filtrate induced necrosis on susceptible calli. The cultivars showed significant variation in callus necrosis. Prolonged duration of selection/screening with CCF totally inhibited the regeneration potential of the calli. Concentrated culture filtrate was not found to inhibit shoot proliferation and shoot growth in already regenerated cultures but inhibited the root growth. In the three direct selection/screening methods tried for calli viz. growing in CCF incorporated MS medium (Method 1) shaking in CCF incorporated liquid MS medium (Method 2) and double layer culture technique (Method 3), cultivars showed significant differences in callus necrosis and callus growth. Direct screening of calli was not found to inhibit the regeneration of shoots, shoot proliferation and recovery of rootable shoots but affected the root growth adversely. Gamma irradiation of calli using 60Co source did not give any better response to in vitro screening. The toxic metabolite(s) present in the culture filtrate could not be separated by organic solvent fractionation. However ion exchangers like Dowex 1 and Dowex 50 could be used for separation of the toxic fraction from the filtrate. The response of five different cultivars at various stages of development of cultures when compared, it was found that the cultivars differed significantly in callusing, callus growth, regeneration of shoots, recovery of rootable shoots and root growth. The clones regenerated from screened and unscreened calli were further tested for resistance/ tolerance to P. capsici using different methods of screening viz.natural screening (keeping in infected field), screening by electrolyte leakage method and screening by artificial inoculation of culture disc of P. capsici. None of the regenerated calliclones were found to be resistant to the disease in natural screening. When the tolerance level of the regenerated calliclones was looked into, the performance of the unscreened calli derived clones was found better as compared to the screened calli derived ones. The calliclones of different cultivars differed significantly inthetolerance/susceptibility reaction to the disease. The calliclones of Cheriakanyakkadan recorded greater degree of tolerance to the disease when compared to others. Among the cultivars studied, Kalluvally exhibited high rate of somaclonal variation.Item Distribution of species of phytophthora affecting coconut and pepper in Kerala(Department of Plant Pathology, College of Agriculture, Vellayani, 1996) Veena, S S; Peethambaran, C KThe present investigation was undertaken to correctly identify the species of Phytophthora causing bud rot of coconut and foot rot of pepper in Kerala and to find out their comparative role in causing diseases on coconut and pepper. The study also aimed at isolation of viable antagonistic agents against the pathogen, so as to utilize them in future for biological control. The study was conducted during 1992-96 at College of Agriculture, Vellayani and Indian Institute of Spices Research, Kozhikode. Detailed symptomatology of both the diseases at different locations were studied. Periodic collections were made from diseased coconut and pepper from seven districts of Kerala. The morphological characters of the isolates were studied in detail. The length, breadth, L/B ratio, pedicel length and caducity of sporangia of coconut isolates were smaller than pepper isolates. The sporangial shapes differed considerably between and among the isolates. All the isolates except those collected from Kannur and Kasaragod districts (C7 - C16), produced abundant number of sporangia on carrot agar. Phytophthora are either heterothallic or homothallic. Generally P. palmivora and P. capsici are heterothallic. But ten coconut isolates obtained from Kannur and Kasaragodu districts produced abundant number of sexual structures on carrot agar, indicating their homothallic nature and this is the first report of homothallic Phytophthora causing bud rot of coconut in Kerala. All other 52 isolates were mated with known A1 and A2 mating type and all the coconut isolates produced oospores with A1 and all the pepper isolates produced oospores when mated with A2. Zoospore production was induced from sporangia using cold treatment. The number of zoospores in sporangia ranged from 6-15 in number. Chlamydlospore production was a rare phenomenon in pepper isolates while all the coconut isolates and five pepper isolates produced chlamydospores. All the isolates were inoculated into pepper seedlings, coconut leaves, tender coconut and coconut seedlings and the time required for infection, variation in symptom expression by different isolates etc. were studied. All the sixteen coconut isolates produced foot rot in pepper. Fourty six isolates of pepper produced lesions on coconut leaves and nut rot in tender coconut, while, only six isolates of pepper produced bud rot in coconut plants. The cross infectivity of pepper isolates needed further confirmation to prove the point beyond doubt. Eight cultivated plants, 9 ornamental plants and 12 weed plants were tested for the host range of coconut and pepper isolates. Many plants belonged to the above three groups took infection successfully. The microorganisms found associated with healthy and diseased pepper and coconut plantations and microorganisms from soils of pepper and coconut gardens of different parts of Kerala were isolated. Out of more than 200 microorganisms, only 28 fungi inhibited the growth of Phytophthora in vitro. On pepper plant, 12 isolates had inhibitory action. Three organisms, viz., Verticillium chlamydosporium, Aspergillus terreus and Chaetomium sp checked the pathogen completely even after seven days of spraying with the organisms.Item Development of diagnosis and recommendation integrated system (DRIS) in black pepper (Piper nigrum L.) in relation to yield and quality characteristics(Department of Agronomy, College of Horticulture, Vellanikkara, 1998) Sreekumaran, V; Neelakandan Potty, NItem Evaluation of nutrient uptake in black pepper (Piper nigrum L .)(Department of Pomology and Floriculture, College of Horticulture, Vellanikkara, 1990) Geetha, C K; Aravindhakshan, MExperiments on the nutritional aspects of black pepper were carried out at the Centre for Advanced Studies on Humid Tropical Tree Crops, College of Horticulture , Vellanikkara and at the Banana Research Station, Kannara, during 1986-1990. The pattern of growth and nutrient- uptake in bush pepper and vine pepper, the relative efficiencies of black pepper varieties in the utilization of applied P and the soil zone of maximum nutrient absorption in pepper vines trailed on dead and live standards were investigated . All the nutrients tried , namely, N, P, K, Ca, Mg and S,had influence on one or more of the growth parameters of bush pepper as well as vine pepper. Application of Ca improved the root characters markedly. Vine pepper had superiority in terms of height, number of leaves and number of roots . Bush pepper produced more number of branches, larger leaves and longer roots. In both the types, the total biomass was a good indicator to applied nutrients . Among the nutrients applied , only N and S increased their concentrations in the stem and leaf of bush pepper and vine pepper. In both the types, treatment devoid of N produced typical N deficiency symptoms. The nature o f response in uptake was more or less similar in all the treatments. The variability in biomass production and nutrient uptake in bush pepper and vine , pepper could be explained by quadratic models for all the nutrients , except Ca. On comparing the quadratic responses In biomass production between the two types of plants, vine pepper revealed an additional response over bush pepper. The differences in nutrient uptake between the two type s of pepper were also highly significant, except for P. Hence, bush pepper cannot be used as a suitable substitute for vine pepper, for purpose o f investigations on nutrient requirements. However, in the context of studies on fertility status and nutrient supplying power of soils , there is scope for using bush pepper as substitute for vine pepper.Item Control of root-knot nematode (Meloidogyne incognita Kofoid and White, Chitwood) infesting black pepper (Piper nigrum L.) by bacterial pathogens(Department of Entomology, College of Horticulture, Vellanikkara, 1990) Sheela, M S; Venkitesan, T SItem Developement of diagnosis and recommentation integrated system(Department of Agronomy, College of Horticulture, Vellanikkara, 1998) Sreekumaran, V; Neelakandan Potty, NItem Investigations on the nutrition of black pepper (Piper nigrum L.)(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 1986) Nybe, E V; Sivaraman Nair, P CItem Fate of carbofuran applied to the soil basin of black pepper(Department of Soil science and Agricultural chemistry, College of Horticulture, Vellanikkara, 1996) Betty Bastin; Wahid, P AAn investigation on the fate of carbofuran applied to the soil basin of black pepper (Piper nigrum L.) was conducted during 1992-1995 at the College of Horticulture, Vellanikkara. Laterite soils collected from the predominant pepper growing tracts of Kannur and Wayanad of Kerala state were used for the study. A field study was also conducted at the KADP farm attached to the College of Horticulture, Vellanikkara. The sorption of carbofuran in laterite soils and its persistence, influence of organic matter on the bio-availability of carbofuran, mobility of the insecticide in soil under field conditions and the effect of carbofuran on the growth of black pepper were assessed. The main findings of the study are given below. The sorption of insecticide in soil was found to be explained by Freundlich adsorption equation. The rates of sorption of carbofuran was not influenced by the organic matter contents . . An initial lag period was observed prior to carbofuran degradation in soil under laboratory conditions. Only one metabolite (3-hydroxycarbofuran) was detected as the product of degradation. The total content of 14C derived radioactivity was high in plants grown in soils with lowest organic matter content and vice versa. Hence, bioavailability of the insecticide is less in soils with high organic matter contents. So a discriminatory application of carbofuran is required depending on organic matter content of the soil. Thus for effective control of the pest in a soil with high organic matter content more quantity or carbofuran has to he applied than a soil with low content of organic matter. The residues of 14C-carbofuran absorbed plants left after HCl extraction, retained significant amounts of radioactivity which could be recovered following combustion. This indicated that determination of extraction efficiency by spiking or fortification of the samples with known quantities of insecticides would lead to overestimation of the recovery. The main metabolite of the carbofuran in the leaves was 3-hydroxy- carbofuran. Autoradiography of the bush pepper plants which was allowed to absorb 14C-carbofuran for 6 h showed that carbofuran was absorbed and translocated to the leaves and berry. Both carbofuran and hydroxycarbofuran were leached to the lower layers of soil (0-60 cm) during SW and NE monsoons. The content of hydroxycarbofuran was less than carbofuran in soils. In the foliage and berries carbofuran and the metabolite, 3-hydroxy- carbofuran accumulated. The content of hydroxycarbofuran was greater than that of carbofuran. As the harvesting time was delayed the content of residues also decreased. The early harvesting and use of immature berries may lead to more concentration of pesticide in the produce. This will lead to chances of more residue build-up in the human system.