PhD Thesis
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Item Genetic analysis of yield and leaf curl virus resistance in chilli(Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2004) Muthuswami, A; Abdul Khader, K MItem Genetic analysis of yield and resistance to anthraconse in chilli(Capsicum annuum L)(Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2004) Ajith, P M; Manju, PItem Disease management and growth improvement in chilli and tomato using Trichoderma Spp. and flurascent pseudomonads(Department of Plant Pathology, College of Agriculture, Vellayani, 2005) Rini, C R; Sulochana, K KItem Genetic analysis of yield and leaf curl virus resistance in chilli (Capsicum spp.)(Department of Plant Breeding, College of Agriculture, Vellayani, 2010) Anandhi, K; Abdul Khader, K MPepper fruits (Capsicum spp.) are among the most consumed vegetables as fresh green or red and dried whole or ground forms in the world for its pungency. Leaf curl virus is an important biotic stress transmitted by the vector, Bemisia tabaci. Controlling the vector can be the only way to manage the disease and results in only partial control of disease. The incidence of disease is more in summer season and makes the cultivation uneconomical. There is an immediate need to develop leaf curl resistant varieties especially for summer cultivation. Hence the present investigation was undertaken at the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during 2007-2009 with the objective of estimating the combining ability, heterosis and gene action involved in the inheritance of yield and leaf curl virus resistance. Five resistant C. frutescens accessions Mangalapuram Local, Thavanur Local, Kayamkulam Local, Mavelikkara Local and Nenmara Local were crossed in L x T pattern with three high yielding susceptible varieties Jwalamukhi, Jwalasakhi and Vellayani Athulya and 15 F1s were produced and evaluated along with the parents in randomized block design. Analysis of variance revealed highly significant genotypic difference for all the characters studied. The observations recorded were plant height (cm), number of branches, number of days to first flowering, plant spread (cm), duration of flowering (fruiting span), number of fruits per plant, fruit length (cm), fruit width (cm), pedicel - fruit ratio, fruit colour at intermediate stage, green fruit yield per plant (g), average fruit weight (g), number of seeds per fruit, hundred seed weight (g), duration of crop, vector population and virus disease scoring. Higher estimates of PCV and GCV were recorded for vulnerability index and green fruit yield per plant. High heritability coupled with high genetic advance were exhibited for days to first flowering, duration of flowering, number of fruits per plant, green fruit yield per plant, number of seeds per fruit, duration of crop and vulnerability index. Combining ability analysis showed that the line Mavelikkara Local and Nenmara Local were alone good general combiners for fruit yield along with leaf curl resistance. Four hybrids viz., Mavelikkara Local x Jwalasakhi, Nenmara Local x Vellayani Athulya, Kayamkulam Local x Jwalamukhi and Thavanur Local x Jwalamukhi exhibited significant sca effect for fruit yield. Considering per se performance, standard heterosis and sca effect two hybrids Mavelikkara Local x Jwalasakhi and Nenmara Local x Vellayani Athulya were found to be superior. Generation mean analysis was carried out using six-parameter model. Six generations viz. P1, P2, F1, F2, B1, B2 were built up among the crosses Mavelikkara Local x Jwalasakhi and Nenmara Local x Vellayani Athulya. Presence of additive, dominance and epistatic interaction for all the characters indicated that hybridization or recombination-breeding programme can be followed for future breeding.Item Triallel analysis of yield and resistance to anthracnose in chilli ( Capsicum annuum L)(Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2007) Haridass, A; Manju, PItem Genetic cataloguing of hot chilli (Capsicum chinese Jacq.)(Department of Olericulture, College of Agriculture,Vellayani, 2001) Manju, P R; Sreelatha Kumary, IThe research project "Genetic cataloguing of hot chilli (Capsicum chinense Jacq.)" was carried out in the vegetable research plot of the Department of Olericulture, College of Agriculture, Vellayani, during September 2000 to May 2001. The objective of the study was to catalogue the accessions based on the IBPGR descriptor for Capsicum and to estimate the genetic parameters for different traits in the germplasm for identifying superior lines based on yield, quality and pest and disease resistance. Thirty two accessions of C. ch inense collected from various sources upon cataloguing pointed out wide variation for several morphological characters. Analysis of variance revealed significant difference among the accessrons for all the characters studied namely, plant height, primary branches per plant, plant spread, days to first flowering, pollen viability, days to maturity, fruits per plant, fruit length, pedicel length, fruit girth, fruit weight, seeds per fruit, 1000-seed weight, yield per plant, yield per harvest, number of harvests, capsaicin, oleoresin, ascorbic acid and mosaic incidence. Among the accessions, CC 23 recorded the maximum yield (1649.72 g) as well as fruits per plant (637.44). CC 13 was the earliest to flower (54.67 days) with the maximum number of harvests (6.94) and least vulnerability index for mosaic (40.63). Fruits per plant recorded the maximum phenotypic and genotypic coefficients of variation, followed by yield per plant and fruit weight. High heritability coupled with high genetic advance was observed for fruits per plant, yield per plant, fruit weight, fruit girth and fruit length. Correlation studies and path coefficient analysis revealed that fruits per plant is the primary yield component as evidenced from its high positive correlation as well as high direct and indirect effects on yield. In the discriminant function analysis, CC 23 (Nemom, Thiruvananthapuram) ranked first, followed by CC 13 (Vithura, Thiruvananthapuram) and CC 7 (Vithura, Thiruvananthapuram). Based on the analysis for genetic divergence, the 32 accessions were grouped into six clusters, with the maximum intercluster distance observed between clusters I and VI. On the basis of the present study, the accessions CC 23, CC 13 and CC 7 were found to be promising with regard to yield, quality and mosaic resistance and the same may be utilized for further improvement.Item Inheritance of clusterness, destalkness and deep red colour in chilli [Capsicum annuum L.](Department of Olericulture, College of Horticulture, Vellanikkara, 1985) Gopalakrishnan, T R; Gopalakrishnan, P KItem Development of chilli (capsicum annuum l.) hybrids with leaf curl virus resistance, high yield and quality(Department of Vegetable Science, College of Agriculture , Vellayani, 2019) Vijeth, S; Sreelathakumary, IItem Bioefficacy and safety evaluation of biorational insecticides for the management of sucking pest complex of chilli (capsicum annuum L. )(Department of Agricultural Entomology, College of Agriculture, Vellayani, 2011) Thania Sara Varghese; Thomas Biju MathewItem Genetic and biochemical bases of resistance to bacterial wilt in chilli(Department Of Olericulture, College Of Horticulture, Vellanikkara, 1996) Baby Lissy Markose; Abdul Vahab, MInvestigation on “Genetic and biochemical bases of resistance to bacterial wilt in chilli’ was carried out in the Department of Olericulture, College of Horticulture, Vellanikkara during 1992-96 to study the inheritance and mechanisms of bacterial wilt resistance and to develop a near isogenic line of Pusa Jwala with resistance to bacterial wilt. Pseudomonas solanacearum, the bacterial wilt pathogen was isolated from infected chilli plants and its pathogenicity was established. The resistance to this disease showed a monogenic and incompletely dominant type of inheritance. Studies on biochemical basis of resistance revealed that total phenol, OD phenol and protein content and enzyme activities had a positive association with bacterial wilt resistance. Ujwala, the resistant variety showed high protein content and had higher activities of peroxidase and polyphenol oxidase enzyme in all plant parts. Higher total phenol was found only in roots and OD phenol in roots and stems of Ujwala. All these biochemical constituents recorded an increasing trend on inoculation in both resistant and subsceptible varieties. The wilt resistant variety had higher OD phenol and protein contents on inoculation whereas the susceptible variety was higher in total phenol content. Both peroxidase and polyphenol oxidase activity were higher in resistant than in susceptible variety. The higher amount of OD phenol and their oxidation products like quinones formed by increased peroxidase and polyphenol oxidase were ascribed for resistance in Ujwala. In the protein gel electrophoresis, the resistant and susceptible genotypes possessed similar bands with identical electrophoretic mobility. The isozyme patterns of peroxidase and esterase were studied. Roots for peroxidase and leaves for esterase showed clear bands. The resistant and susceptible varieties were different in banding pattern before inoculation. Inoculation did not evince any change in the banding pattern for peroxidase and esterase enzymes. The resistant variety had a well developed secondary xylem with thick and compact piliferous layers and cortical cells compared to the susceptible variety. In the radiotracer studies, radioactivity counts were more in susceptible than in resistant variety after inoculation in both 32p and 35S labeled plants. In the autoradiography, the 35S labeled bacteria were more in susceptible plants than in resistant ones. However there was no appreciable difference between resistant and susceptible plants when 32P tagged bacteria were inoculated. A near isogenic line of Pusa Jwala, moderately resistant to bacterial wilt, was developed by crossing Ujwala with Pusa Jwala and advancing the F1s to BC2F4 generation.